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GRPR antibody [C1C2], Internal

Anti-GRPR antibody [C1C2], Internal used in Western Blot (WB). GTX100015
Anti-GRPR antibody [C1C2], Internal used in Western Blot (WB). GTX100015
Anti-GRPR antibody [C1C2], Internal used in Western Blot (WB). GTX100015
Anti-GRPR antibody [C1C2], Internal used in Western Blot (WB). GTX100015

Cat. No. GTX100015

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all GRPR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

HeLaS3 , Molt-4 , mouse pancreas , mouse brain , rat brain , rat pancreas

Predict Reactivity

Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human GRPR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240922

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

gastrin releasing peptide receptor , BB2 , BB2R

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

Gastrin-releasing peptide (GRP) regulates numerous functions of the gastrointestinal and central nervous systems, including release of gastrointestinal hormones, smooth muscle cell contraction, and epithelial cell proliferation and is a potent mitogen for neoplastic tissues. The effects of GRP are mediated through the gastrin-releasing peptide receptor. This receptor is a glycosylated, 7-transmembrane G-protein coupled receptor that activates the phospholipase C signaling pathway. The receptor is aberrantly expressed in numerous cancers such as those of the lung, colon, and prostate. An individual with autism and multiple exostoses was found to have a balanced translocation between chromosome 8 and a chromosome X breakpoint located within the gastrin-releasing peptide receptor gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GRPR antibody [C1C2], Internal used in Western Blot (WB). GTX100015

GTX100015 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GRPR antibody [C1C2], Internal (GTX100015) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GRPR antibody [C1C2], Internal used in Western Blot (WB). GTX100015

GTX100015 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GRPR antibody [C1C2], Internal (GTX100015) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GRPR antibody [C1C2], Internal used in Western Blot (WB). GTX100015

GTX100015 WB Image

Sample(30 μg of whole cell lysate)
A:HeLa S3(GTX14654)
B:MOLT4(GTX27912)
10% SDS PAGE
GTX100015 diluted at 1:1000

Anti-GRPR antibody [C1C2], Internal used in Western Blot (WB). GTX100015

GTX100015 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GRPR antibody [C1C2], Internal (GTX100015) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Takanami K et al. J Comp Neurol 2022; 530 (16) : 2804-2819 Characterization of the expression of gastrin-releasing peptide and its receptor in the trigeminal and spinal somatosensory systems of Japanese macaque monkeys: Insight into humans.
Takanami K et al. JSHC 2016; 49 (6) : 181-190 Comparative Anatomy of Gastrin-releasing Peptide Pathways in the Trigeminal Sensory System of Mouse and the Asian House Musk Shrew Suncus murinus.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 399
$ 169