GSK3 alpha (phospho Ser21) + GSK3 beta (phospho Ser9) antibody

Name: GSK3 alpha (phospho Ser21) + GSK3 beta (phospho Ser9) antibody
Brand: GeneTex
SKU: GTX132997
Price: 399.00 USD
Rating: 3.5 (2 reviews)

Anti-GSK3 alpha (phospho Ser21) + GSK3 beta (phospho Ser9) antibody used in Western Blot (WB). GTX132997

Cat. No. GTX132997

Reference 5

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-Fr
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

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View product citations for antibody GTX132997 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-Fr	Assay dependent

Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

NIH3T3( starvation for 16 hr and 5 ng/ml PDGF treatment for 15 min)

Predict Reactivity

Rat, Sheep, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.13 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser9 of human GSK3 beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886803

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glycogen synthase kinase 3 beta

Background

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase (see GYS1, MIM 138570). Two isoforms, alpha (GSK3A; MIM 606784) and beta, show a high degree of amino acid homology (Stambolic and Woodgett, 1994 [PubMed 7980435]). GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation (Plyte et al., 1992 [PubMed 1333807]).[supplied by OMIM]

Database

Gene ID: 2932 GSK3B
UniProt: P49841 GSK3B

Research Area

DATA IMAGES

GTX132997 WB Image

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (18 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 alpha (phospho Ser21) + GSK3 beta (phospho Ser9) antibody (GTX132997) diluted at 1:1000.

REFERENCE

Application Reference

Hung SY et al. Front Cell Neurosci 2022; 16 : 880267 Electroacupuncture improves TBI dysfunction by targeting HDAC overexpression and BDNF-associated Akt/GSK-3β signaling.

Liu Y.J. et al. Int. J. Mol. Sci. 2021; 22 (6) : 2823 The Anti-Melanogenesis Effect of 3,4-Dihydroxybenzalacetone through Downregulation of Melanosome Maturation and Transportation in B16F10 and Human Epidermal Melanocytes.

Application : WB

Zuo Z et al. Cell Biol Int 2020; 44 (11) : 2284-2292 LncRNA TTN-AS1/miR-134-5p/PAK3 axis regulates the radiosensitivity of human large intestine cancer cells through the P21 pathway and AKT/GSK-3β/β-catenin pathway.

Application : WB

Liu J et al. Oncol Lett 2017; 14 (5) : 5711-5718 miR-214 targets the PTEN-mediated PI3K/Akt signaling pathway and regulates cell proliferation and apoptosis in ovarian cancer.

Leibinger M et al. Proc Natl Acad Sci U S A 2017; 114 (27) : E5454-E5463 Boosting CNS axon regeneration by harnessing antagonistic effects of GSK3 activity.

Application : IHC-Fr

Reactivity : Mouse

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