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Galectin 1 antibody

KO/KD Orthogonal
Anti-Galectin 1 antibody used in Western Blot (WB). GTX101566
Anti-Galectin 1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX101566
Anti-Galectin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101566
Anti-Galectin 1 antibody used in Western Blot (WB). GTX101566
Anti-Galectin 1 antibody used in Western Blot (WB). GTX101566
Anti-Galectin 1 antibody used in Western Blot (WB). GTX101566
Anti-Galectin 1 antibody used in Western Blot (WB). GTX101566
Anti-Galectin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101566

Cat. No. GTX101566

Reference 11
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Galectin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IHC-Wm Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

NCI-H929 , BCL-1 , PC-12 , Rat2 , HeLa

Predict Reactivity

Sheep, Bovine, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.52 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Galectin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11177712

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

galectin 1 , GAL1 , GBP

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Galectin 1 antibody used in Western Blot (WB). GTX101566

GTX101566 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin 1 antibody (GTX101566) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Galectin 1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX101566

GTX101566 IHC-Fr Image

Galectin 1 antibody detects Galectin 1 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Galectin 1 protein stained by Galectin 1 antibody (GTX101566) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-Galectin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101566

GTX101566 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Galectin 1(GTX101566) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Galectin 1 antibody used in Western Blot (WB). GTX101566

GTX101566 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin 1 antibody (GTX101566) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Galectin 1 antibody used in Western Blot (WB). GTX101566

GTX101566 WB Image

Sample (30 μg of whole cell lysate)
A: NCI-H929
15% SDS PAGE
GTX101566 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Galectin 1 antibody used in Western Blot (WB). GTX101566

GTX101566 WB Image

Sample (30 μg of whole cell lysate)
A: BCL-1
15% SDS PAGE
GTX101566 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Galectin 1 antibody used in Western Blot (WB). GTX101566

GTX101566 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin 1 antibody (GTX101566) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Galectin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101566

GTX101566 ICC/IF Image

Galectin 1 antibody detects Galectin 1 protein at cytoplasm by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Galectin 1 stained by Galectin 1 antibody (GTX101566) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

REFERENCE

Application Reference

Tarighat SS et al. Int J Mol Sci 2021; 22 (22) Overcoming Microenvironment-Mediated Chemoprotection through Stromal Galectin-3 Inhibition in Acute Lymphoblastic Leukemia.
Application : WB
Reactivity : Human
Russo A.J. et al. Nat Immunol. 2021; 22 (2) : 154-165 Intracellular immune sensing promotes inflammation via gasdermin D-driven release of a lectin alarmin.
Application : WB
Reactivity : Mouse
Borowski S et al. Front Immunol 2020; 11 : 1316 Altered Glycosylation Contributes to Placental Dysfunction Upon Early Disruption of the NK Cell-DC Dynamics.
Application : IHC-Fr
Reactivity : Mouse
Chen JF et al. J Pharm Biomed Anal 2020; 187 : 113142 Proteomic analysis of Antrodia Cinnamomea-induced ER stress in liver cancer cells.
Application : WB
Reactivity : Human
Paz H et al. J Exp Clin Cancer Res 2018; 37 (1) : 67 Treatment of B-cell precursor acute lymphoblastic leukemia with the Galectin-1 inhibitor PTX008.
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Submit a Reference

REVIEW

Galectin 1 antibody Cat. No. GTX101566

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-Galectin 1 antibody used in Western Blot (WB). GTX101566
Date : Anonymous submitted on 07-Jul-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : U2OS cells
Amount used : 10μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Submit a Review
Application Reference
Tarighat SS et al. Int J Mol Sci 2021; 22 (22) Overcoming Microenvironment-Mediated Chemoprotection through Stromal Galectin-3 Inhibition in Acute Lymphoblastic Leukemia.
Application : WB
Reactivity : Human
Russo A.J. et al. Nat Immunol. 2021; 22 (2):154-165 Intracellular immune sensing promotes inflammation via gasdermin D-driven release of a lectin alarmin.
Application : WB
Reactivity : Mouse
Borowski S et al. Front Immunol 2020; 11:1316 Altered Glycosylation Contributes to Placental Dysfunction Upon Early Disruption of the NK Cell-DC Dynamics.
Application : IHC-Fr
Reactivity : Mouse
Chen JF et al. J Pharm Biomed Anal 2020; 187:113142 Proteomic analysis of Antrodia Cinnamomea-induced ER stress in liver cancer cells.
Application : WB
Reactivity : Human
Paz H et al. J Exp Clin Cancer Res 2018; 37 (1):67 Treatment of B-cell precursor acute lymphoblastic leukemia with the Galectin-1 inhibitor PTX008.
Li JM et al. Ther Adv Med Oncol 2018; 10:1.76E+15 Upregulation of LGALS1 is associated with oral cancer metastasis.
Application : WB, IHC-P
Reactivity : Human
Kolobovnikova YV et al. Bull Exp Biol Med 2018; (Epub) Expression of Galectins-1 and Galectin-3 in Stomach and Colorectal Cancer with Tissue Eosinophilia.
Application : IHC-P
Reactivity : Human
You JL et al. Mol Cell Endocrinol 2017; (Epub) A potential role of galectin-1 in promoting mouse trophoblast stem cell differentiation.
Application : IHC-Wm
Reactivity : Mouse
Barbe C et al. J Proteome Res 2017; (Epub) Comparative Proteomic and Transcriptomic Analysis of Follistatin-induced Skeletal Muscle Hypertrophy.
Application : WB
Reactivity : Mouse
Sundararaman B et al. Mol Cell 2016; 61 (6):903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : IP
Reactivity : Human
Brandi J et al. J Proteomics 2016; 136:43112 Secretome protein signature of human pancreatic cancer stem-like cells.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169