GeneTex
United States (US)

Galectin 3 antibody

KO/KD Overexpression
GTX125897_40863_20170406_WB_KO_watermark_18121410_769.jpg
GTX125897_40863_20190201_WB_19022001_304.jpg
GTX125897_40863_WB_18121410_980.jpg
GTX125897_40863_WB_B_18121410_345.jpg
GTX125897_40863_WB_R_colon_18121410_837.jpg
GTX125897_40975_IHC_18121410_708.jpg

Cat No. GTX125897

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
ELISA Assay dependent
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

HeLa , A549 , HCT116 , 293T transfected recombinant LGALS3 protein , rat colon

Predict Reactivity

Rabbit, Bovine, Dog, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Galectin3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CBP35 antibody, GAL3 antibody, GALBP antibody, GALIG antibody, L31 antibody, LGALS2 antibody, MAC2 antibody, LGALS3 antibody, IgE-binding protein antibody, carbohydrate-binding protein 35 antibody, 35 kDa lectin antibody, MAC-2 antigen antibody, galactose-specific lectin 3 antibody, galectin-3 antibody, laminin-binding protein antibody, lectin L-29 antibody, "lectin, galactoside-binding, soluble, 3 antibody"

Cellular Localization

Nucleus

Background

This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. Alternate splicing results in multiple transcript variants.

Database

Research Area

DATA IMAGES
GTX125897_40863_20170406_WB_KO_watermark_18121410_769.jpg

GTX125897 WB Image

KO/KD

Wild-type (WT) and Galectin 3 knockout (KO) HeLa cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody (GTX125897) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
CRISPR knockout cell lines generated by www.edigene.com

GTX125897_40863_20190201_WB_19022001_304.jpg

GTX125897 WB Image

Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody (GTX125897) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX125897_40863_WB_18121410_980.jpg

GTX125897 WB Image

Sample (30 ug of whole cell lysate)
A: A549
B: HCT116
12% SDS PAGE
GTX125897 diluted at 1:1000

GTX125897_40863_WB_B_18121410_345.jpg

GTX125897 WB Image

Overexpression

Sample (30 ug of whole cell lysate)
A: Non-transfected 293T lysate
B: LGALS3 transfected 293T lysates
12% SDS PAGE
GTX125897 diluted at 1:1000

GTX125897_40863_WB_R_colon_18121410_837.jpg

GTX125897 WB Image

Galectin3 antibody detects Galectin3 protein by western blot analysis.
A. 50 μg rat colon lysate/extract
12 % SDS-PAGE
Galectin3 antibody (GTX125897) dilution: 1:1000

GTX125897_40975_IHC_18121410_708.jpg

GTX125897 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using Galectin3 antibody(GTX125897) antibody at 1:200 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW

Galectin 3 antibody Cat No. GTX125897

Rating ( Average 5 based on 3 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 13-Aug-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : SAS cells
Primary Antibody : 1:1000, 4°C, overnightHr
Date : Anonymous submitted on 24-Mar-2017
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Liver tissue
Primary Antibody : 1:200, 4°C, overnightHr
Figure Note : control/diabetic mouse (250x lens)
Date : Anonymous submitted on 24-Mar-2017
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Liver tissue
Primary Antibody : 1:200, 4°C, overnightHr
Figure Note : control/diabetic mouse (400x lens)
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169