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Gfap antibody

Anti-Gfap antibody used in Western Blot (WB). GTX128644
Anti-Gfap antibody used in IHC (Whole mount) (IHC-Wm). GTX128644

Cat. No. GTX128644

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Wm

Reactivity

 Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all zebrafish Gfap products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-Wm 1:100-1:500
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

24hpf zebrafish embryos

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.87 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Recombinant protein encompassing a sequence within the center region of zebrafish GFAP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885805

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glial fibrillary acidic protein , cb345 , etID36982.3 , gfapl , wu:fb34h11 , wu:fk42c12 , xx:af506734 , zgc:110485

Cellular Localization

Cytoplasm , cytoskeleton

Database

Research Area

DATA IMAGES

Anti-Gfap antibody used in Western Blot (WB). GTX128644

GTX128644 WB Image

Gfap antibody detects Gfap protein by western blot analysis. Zebrafish tissue extracts (30 μg) was separated by 10 % SDS-PAGE, and the membrane was blotted with Gfap antibody (GTX128644) at a dilution of 1:1000.

Anti-Gfap antibody used in IHC (Whole mount) (IHC-Wm). GTX128644

GTX128644 IHC-Wm Image

Gfap antibody detects Gfap protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Gfap antibody (GTX128644) dilution: 1:100.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169