APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:500-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HeLa
Predict Reactivity
Mouse, Rat, Rabbit, Sheep, Bovine, Cat, Dog, Guinea pig, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.38 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Glucocorticoid Receptor. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10721056
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
nuclear receptor subfamily 3 group C member 1 , GCCR , GCR , GCRST , GR , GRL
Cellular Localization
Cytoplasm , Nucleus
Background
The protein encoded by this gene is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. This protein can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Mutations in this gene are a cause of glucocorticoid resistance, or cortisol, resistance. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX113208 IP Image
Glucocorticoid receptor antibody immunoprecipitates Glucocorticoid receptor protein in IP experiments. IP Sample: 1000 μg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of Glucocorticoid receptor protein by 2.5 μg of Glucocorticoid receptor antibody (GTX113208) 7.5% SDS-PAGE The immunoprecipitated Glucocorticoid receptor protein was detected by Glucocorticoid receptor antibody (GTX113208) diluted at 1:1000.
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GTX113208 WB Image
Wild-type (WT) and Glucocorticoid receptor knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glucocorticoid receptor antibody (GTX113208) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX113208 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Glucocorticoid Receptor antibody (GTX113208) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113208 ICC/IF Image
Glucocorticoid Receptor antibody detects Glucocorticoid Receptor protein at nucleus by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Glucocorticoid Receptor stained by Glucocorticoid Receptor antibody (GTX113208) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX113208 IHC-P Image
Glucocorticoid Receptor antibody detects Glucocorticoid Receptor protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.Glucocorticoid Receptor stained by Glucocorticoid Receptor antibody (GT
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GTX113208 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Glucocorticoid receptor(GTX113208) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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REFERENCE
There are currently no references for Glucocorticoid Receptor antibody (GTX113208). Be the first to share your publications with this product.
REVIEW
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