GeneTex
United States (US)

Glutamate Dehydrogenase antibody, Internal

GTX88164_2489_WB_18121411_833.jpg
GTX88164_1043_IHC-P_18121411_825.jpg
GTX88164_2488_WB_18122102_984.jpg

Cat No. GTX88164

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.3-1μg/ml
ICC/IF Assay dependent
IHC-P 3-5μg/ml

Note :

IHC-P
Human Cerebral Cortex shows mitochondrial staining in neuronal cell bodies and axons.

Not tested in other applications.

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88164-PEP).

Specificity/Sensitivity

This antibody is expected to recognize both reported isoforms (NP_005262.1; NP_036216.2).

Predict Reactivity

Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-ESEEQKRNRVRGILR, from the internal region of the protein sequence according to NP_005262.1; NP_036216.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
GTX88164_2489_WB_18121411_833.jpg

GTX88164 WB Image

WB analysis of human (A) and mouse (B) brain lysate using GTX88164 Glutamate Dehydrogenase antibody, Internal.
Dilution : 0.03µg/ml
Loading : 35µg protein in RIPA buffer

GTX88164_1043_IHC-P_18121411_825.jpg

GTX88164 IHC-P Image

IHC-P analysis of human cerebral cortex using GTX88164 Glutamate Dehydrogenase antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8µg/ml

GTX88164_2488_WB_18122102_984.jpg

GTX88164 WB Image

WB analysis of human (A), mouse (B) and rat (C) liver lysate using GTX88164 Glutamate Dehydrogenase antibody, Internal.
Dilution : 0.01µg/ml
Loading : 35µg protein in RIPA buffer

REFERENCE
Package List Price ($)
$ 329