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GLUD1 + GLUD2 antibody, Internal

Anti-GLUD1 + GLUD2 antibody, Internal used in Western Blot (WB). GTX88164
Anti-GLUD1 + GLUD2 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88164
Anti-GLUD1 + GLUD2 antibody, Internal used in Western Blot (WB). GTX88164
Anti-GLUD1 + GLUD2 antibody, Internal used in Western Blot (WB). GTX88164

Cat. No. GTX88164

Reference 1

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all Glutamate Dehydrogenase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.3-1μg/ml
ICC/IF Assay dependent
IHC-P 3-5μg/ml

Note :

IHC-P
Human Cerebral Cortex shows mitochondrial staining in neuronal cell bodies and axons.
Not tested in other applications.

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88164-PEP).

Product Note

This antibody is expected to recognize both reported isoforms (NP_005262.1; NP_036216.2).

Predict Reactivity

Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-ESEEQKRNRVRGILR, from the internal region of the protein sequence according to NP_005262.1; NP_036216.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

RRID

AB_10722538

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-GLUD1 + GLUD2 antibody, Internal used in Western Blot (WB). GTX88164

GTX88164 WB Image

WB analysis of human (A) and mouse (B) brain lysate using GTX88164 GLUD1 + GLUD2 antibody, Internal.
Dilution : 0.03μg/ml
Loading : 35μg protein in RIPA buffer

Anti-GLUD1 + GLUD2 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88164

GTX88164 IHC-P Image

IHC-P analysis of human cerebral cortex using GTX88164 GLUD1 + GLUD2 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8μg/ml

Anti-GLUD1 + GLUD2 antibody, Internal used in Western Blot (WB). GTX88164

GTX88164 WB Image

WB analysis of human (A), mouse (B) and rat (C) liver lysate using GTX88164 GLUD1 + GLUD2 antibody, Internall.
Dilution : 0.01μg/ml
Loading : 35μg protein in RIPA buffer

Anti-GLUD1 + GLUD2 antibody, Internal used in Western Blot (WB). GTX88164

GTX88164 WB Image

The data was published in the journal Cancer Metab in 2013. PMID: 24304688

REFERENCE

Application Reference

McGuirk S et al. Cancer Metab 2013; 1 (1) : 22 PGC-1α supports glutamine metabolism in breast cancer.
Application : WB
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399