GLUD1 + GLUD2 antibody

Name: GLUD1 + GLUD2 antibody
Brand: GeneTex
SKU: GTX105765
Price: 399.00 USD
Rating: 4 (2 reviews)

Anti-GLUD1 + GLUD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105765

Anti-GLUD1 + GLUD2 antibody used in Western Blot (WB). GTX105765

Anti-GLUD1 + GLUD2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105765

Cat. No. GTX105765

Reference 3

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, ELISA
Reactivity	Human, Mouse, Rat, Drosophila
Package	100 μl ($399), 25 μl ($169)

See all Glutamate Dehydrogenase products

View product citations for antibody GTX105765 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
ELISA	Assay dependent

Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Sheep, Bovine, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.07 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Glutamate Dehydrogenase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11173313

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutamate dehydrogenase 1 , GDH , GDH1 , GLUD

Cellular Localization

Mitochondrion matrix

Background

L-glutamate dehydrogenase (EC 1.4.1.3) has a central role in nitrogen metabolism in plants and animals. Glutamate dehydrogenase is found in all organisms and catalyzes the oxidative deamination of 1-glutamate to 2-oxoglutarate (Smith et al., 2001 [PubMed 11254391]). Glutamate, the main substrate of GLUD, is present in brain in concentrations higher than in other organs. In nervous tissue, GLUD appears to function in both the synthesis and the catabolism of glutamate and perhaps in ammonia detoxification (Mavrothalassitis et al., 1988 [PubMed 3368458]).[supplied by OMIM]

Database

Gene ID: 2746 GLUD1
UniProt: P00367 GLUD1

Research Area

DATA IMAGES

GTX105765 IHC-P Image

GLUD1 + GLUD2 antibody detects Glutamate Dehydrogenase protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Glutamate Dehydrogenase stained byGLUD1 + GLUD2 antibody (GTX105765) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX105765 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GLUD1 + GLUD2 antibody (GTX105765) diluted at 1:1000.

GTX105765 ICC/IF Image

GLUD1+ GLUD2 antibody detects Glutamate Dehydrogenase protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Glutamate Dehydrogenase protein stained by GLUD1 + GLUD2 antibody (GTX105765) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX105765 WB Image

Drosophila tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GLUD1 + GLUD2 antibody (GTX105765) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105765 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using GLUD1 + GLUD2 antibody (GTX105765) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105765 ICC/IF Image

GLUD1 + GLUD2 antibody detects Glutamate Dehydrogenase protein at cytoplasm by immunofluorescent analysis.
Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Glutamate Dehydrogenase protein stained by GLUD1 + GLUD2 antibody (GTX105765) diluted at 1:500.
Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody (GTX631836) diluted at 1:200.
Blue: Hoechst 33342 staining.

GTX105765 IHC-P Image

GLUD1 + GLUD2 antibody detects Glutamate Dehydrogenase protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Glutamate Dehydrogenase stained by GLUD1 + GLUD2 antibody (GTX105765) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX105765 IHC-P Image

The data was published in the journal Sci Rep in 2018. PMID: 29555943

REFERENCE

Application Reference

Geiszler PC et al. Sci Rep 2018; 8 (1) : 4833 Dynamic metabolic patterns tracking neurodegeneration and gliosis following 26S proteasome dysfunction in mouse forebrain neurons.

Application : IHC-P

Reactivity : Mouse

Gonzalez SN et al. PLoS One 2017; 12 (2) : e0172405 Structure, kinetic characterization and subcellular localization of the two ribulose 5-phosphate epimerase isoenzymes from Trypanosoma cruzi.

Application : WB

Cho CK et al. J Proteome Res 2010; 9 (7) : 3574-82 Amniotic fluid proteome analysis from Down syndrome pregnancies for biomarker discovery.

Application : ELISA

Reactivity : Human

Submit a Reference

REVIEW