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Glutaminase C antibody

Anti-Glutaminase C antibody used in IHC (Paraffin sections) (IHC-P). GTX131263
Anti-Glutaminase C antibody used in IHC (Paraffin sections) (IHC-P). GTX131263
Anti-Glutaminase C antibody used in IHC (Paraffin sections) (IHC-P). GTX131263
Anti-Glutaminase C antibody used in Immunoprecipitation (IP). GTX131263
Anti-Glutaminase C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131263
Anti-Glutaminase C antibody used in Western Blot (WB). GTX131263

Cat No. GTX131263

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

MDA-MB-231 , *cholangiocarcinoma tissue

Specificity/Sensitivity

Specific for GAC but not KGA isoform

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Glutaminase C. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Glutaminase,Aad20,Gac,Gam,Gls1,Kga,Gls

Cellular Localization

Mitochondrion

Background

Sahai (1983) [PubMed 6825316] demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter (Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.[supplied by OMIM]

Database

Research Area

DATA IMAGES
Anti-Glutaminase C antibody used in IHC (Paraffin sections) (IHC-P). GTX131263

GTX131263 IHC-P Image

Glutaminase C antibody detects Glutaminase C protein at cytoplasm on mouse duidenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duidenum.
Glutaminase C antibody (GTX131263) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Glutaminase C antibody used in IHC (Paraffin sections) (IHC-P). GTX131263

GTX131263 IHC-P Image

Glutaminase C antibody detects Glutaminase C protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Glutaminase C antibody (GTX131263) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Glutaminase C antibody used in IHC (Paraffin sections) (IHC-P). GTX131263

GTX131263 IHC-P Image

Glutaminase C antibody detects Glutaminase C protein at cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
Glutaminase C antibody (GTX131263) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Glutaminase C antibody used in Immunoprecipitation (IP). GTX131263

GTX131263 IP Image

Immunoprecipitation of Glutaminase C protein from MDA-MB-231 whole cell extracts using 5 μg of Glutaminase C antibody (GTX131263) or Glutaminase C antibody (GTX132402).
Western blot analysis was performed using Glutaminase C antibody (GTX131263).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Glutaminase C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131263

GTX131263 ICC/IF Image

Glutaminase C antibody detects Glutaminase C protein at mitochondria by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Glutaminase C protein stained by Glutaminase C antibody (GTX131263) diluted at 1:500.
Red: Histone H3K9ac (acetyl Lys9), a nucleus marker, stained by Histone H3K9ac (acetyl Lys9) antibody [GT464] (GTX630554) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Glutaminase C antibody used in Western Blot (WB). GTX131263

GTX131263 WB Image

Glutaminase C (GAC) antibody validation by shRNA knock-down. Non-transfected (-) and GLS1(GAC isoform specific) shRNA-transfected MDA-MB-231 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glutaminase C (GAC) antibody (GTX131263) at a dilution of 1:5000.
This antibody specifically recognizes GAC but not the KGA isoform

REFERENCE
REVIEW

Glutaminase C antibody Cat No. GTX131263

Rating ( Average 4 based on 1 users reviews)
Application
IHC (Paraffin sections)(IHC-P) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 19-Mar-2015
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : cholangiocarcinoma tissue
Primary Antibody : 1:500, 4°C, overnightHr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169