Glypican 4 antibody [AT51E3]

Cat. No. GTX16498

Host	Mouse
Clonality	Monoclonal
Clone Name	AT51E3
Isotype	IgG2a
Application	WB, ICC/IF, FACS, ELISA
Reactivity	Human
Package	100 μl ($399)

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Datasheet
Document

View product citations for antibody GTX16498 on CiteAb

APPLICATION

Application Note

WB: 1:1000. ICC/IF: 1:200. FACS: 1:200

Calculated MW

62 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human GPC4 (401-529aa)

Purification

By protein-A affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glypican 4 , K-glypican

Cellular Localization

Cell membrane

Background

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The GPC4 gene is adjacent to the 3' end of GPC3 and may also play a role in Simpson-Golabi-Behmel syndrome. [provided by RefSeq, Jul 2008]

Database

Gene ID: 2239 GPC4
UniProt: O75487 GPC4

Research Area

DATA IMAGES

GTX16498 FACS Image

FACS analysis of 293T cells using an isotype control (black) or GPC4 antibody [AT51E3] at 2-5ug for 1,000,000 cells (red).

GTX16498 ICC/IF Image

ICC/IF analysis of 293T cells using DAPI (blue) and GPC4 antibody [AT51E3] at a dilution of 1:200 (green).

GTX16498 WB Image

WB analysis of (1) recombinant human GPC4 (50ng) and (2) Ramos lysate (40ug) using GPC4 antibody [AT51E3] at a dilution of 1:1000.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty