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HARS antibody [N1C1]

Anti-HARS antibody [N1C1] used in Western Blot (WB). GTX114032
Anti-HARS antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX114032

Cat. No. GTX114032

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all HARS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

HepG2 , HCT116

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.89 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HARS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620338

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

histidyl-tRNA synthetase , CMT2W , HRS , USH3B

Cellular Localization

Cytoplasm

Background

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HARS antibody [N1C1] used in Western Blot (WB). GTX114032

GTX114032 WB Image

HARS antibody [N1C1] detects HARS protein by Western blot analysis.
A. 30 μg HepG2 whole cell lysate/extract
B. 30 μg HCT116 whole cell lysate/extract
7.5 % SDS-PAGE
HARS antibody [N1C1] (GTX114032) dilution: 1:500

Anti-HARS antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX114032

GTX114032 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using HARS(GTX114032) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169