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HAUS2 antibody [N1C3-2]

Anti-HAUS2 antibody [N1C3-2] used in Western Blot (WB). GTX118734
Anti-HAUS2 antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118734
Anti-HAUS2 antibody [N1C3-2] used in Western Blot (WB). GTX118734
Anti-HAUS2 antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118734

Cat. No. GTX118734

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all HAUS2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human HAUS2 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624937

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

HAUS augmin like complex subunit 2 , C15orf25 , CEP27 , HsT17025

Cellular Localization

Centrosome , Spindle

Background

HAUS2 is 1 of 8 subunits of the 390-kD human augmin complex, or HAUS complex. The augmin complex was first identified in Drosophila, and its name comes from the Latin verb 'augmentare,' meaning 'to increase.' The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly (Goshima et al., 2008 [PubMed 18443220]; Uehara et al., 2009 [PubMed 19369198]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-HAUS2 antibody [N1C3-2] used in Western Blot (WB). GTX118734

GTX118734 WB Image

IP-WB assay to show that Hice1 (GTX113617) co-immunoprecipitated with other Augmin components HAUS6 (GTX118732), HAUS2 (GTX118734) and HAUS1 (GTX118733) in U2OS cells.

Anti-HAUS2 antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118734

GTX118734 ICC/IF Image

HAUS2 antibody [N1C3-2] detects HAUS2 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HAUS2 protein stained by HAUS2 antibody [N1C3-2] (GTX118734) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-HAUS2 antibody [N1C3-2] used in Western Blot (WB). GTX118734

GTX118734 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
12% SDS PAGE
GTX118734 diluted at 1:1000

Anti-HAUS2 antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118734

GTX118734 ICC/IF Image

HAUS2 antibody [N1C3-2] detects HAUS2 protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: HAUS2 protein stained by HAUS2 antibody [N1C3-2] (GTX118734) diluted at 1:500.
Blue: Hoechst 33343 staining.

REFERENCE

Application Reference

Ustinova K et al. J Cell Sci 2023; 136 (12) Microtubule binding of the human augmin complex is directly controlled by importins and Ran-GTP.
Tsai CY et al. J Biol Chem 2011; 286 (34) : 30097-106 Aurora-A phosphorylates Augmin complex component Hice1 protein at an N-terminal serine/threonine cluster to modulate its microtubule binding activity during spindle assembly.
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169