GeneTex
United States (US)

HIF1 alpha antibody [GT10211]

Orthogonal
GTX628480_41337_20150417_WB_CoCl2_18121411_748.jpg
GTX628480_41134_CT_IFA_18121411_252.jpg
GTX628480_41134_WB_Hypoxia_18121411_314.jpg
GTX628480_41134_IP_18121411_840.jpg

Cat No. GTX628480

Host Mouse
Clonality Monoclonal
Clone Name GT10211
Isotype IgG2a
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:100-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IP 1:100-1:500
Not tested in other applications.

Calculated MW

93 kDa. ( Note )

Positive Control

HepG2 (hypoxia:1%O2 48hr)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The immunogen used to generate this antibody corresponds to human HIF1 alpha

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HIF-1alpha antibody, HIF1 antibody, HIF1-ALPHA antibody, MOP1 antibody, PASD8 antibody, bHLHe78 antibody, HIF1A antibody, basic-helix-loop-helix-PAS protein MOP1 antibody, class E basic helix-loop-helix protein 78 antibody, ARNT-interacting protein antibody, member of PAS superfamily 1 antibody, hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) antibody, hypoxia-inducible factor 1-alpha antibody, hypoxia-inducible factor 1 alpha isoform I.3 antibody, PAS domain-containing protein 8 antibody, member of PAS protein 1 antibody, HIF-1-alpha antibody, ARNT interacting protein antibody, hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) antibody

Cellular Localization

Cytoplasm , Nucleus

Background

Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX628480_41337_20150417_WB_CoCl2_18121411_748.jpg

GTX628480 WB Image

Orthogonal

HIF1 alpha antibody [GT10211] detects HIF1 alpha protein by western blot analysis. Un-treated (-) and treated (+, 500 μM CoCl2 treatment for 24hr) HeLa whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody [GT10211] (GTX628480) at a dilution of 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX628480_41134_CT_IFA_18121411_252.jpg

GTX628480 ICC/IF Image

HIF1 alpha antibody detects HIF1 alpha protein at nuclear by confocal immunofluorescent analysis. Sample: Hypoxia (500μM CoCl2) treated 24hr (right) or untreated (left) HeLa cells were fixed in 4% paraformaldehyde for 15 min. Red: HIF1 alpha protein stained by HIF1 alpha antibody (GTX628480) diluted at 1:250. Green: Alpha-tubulin, a cytoskeleton marker, stained by Rabbit Polyclonal antibody (GTX102078) diluted at 1:500. [Images captured by Olympus FV10i Confocal Laser Scanning Microscope]

GTX628480_41134_WB_Hypoxia_18121411_314.jpg

GTX628480 WB Image

Orthogonal

HIF1 alpha antibody [GT10211] detects HIF1A protein by western blot analysis.
A. 30 µg HepG2 whole cell lysate/extract (untreated)
B. 30 µg HepG2 whole cell lysate/extract (1%O2 treatment for 48hr)
7.5% SDS-PAGE
HIF1 alpha antibody [GT10211] (GTX628480) dilution: 1:250
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX628480_41134_IP_18121411_840.jpg

GTX628480 IP Image

HIF-1 alpha antibody immunoprecipitates HIF-1 alpha protein in IP experiments.
IP Sample: 1000 µg HepG2 whole cell lysate/extract
A. 40 μg HepG2 whole cell lysate/extract from normoxia
B. 40 μg HepG2 whole cell lysate/extract from hypoxia 24hr (1%O2)
C. Control with 2.5 μg of preimmune mouse IgG/HepG2 whole cell
lysate/extract from normoxia
D. Control with 2.5 μg of preimmune mouse IgG/ HepG2 whole cell
lysate/extract from hypoxia 24hr (1%O2)
E. Immunoprecipitation of HIF-1 alpha protein by 2.5 μg of HIF-1
alpha antibody (GTX628480)/HepG2 whole cell lysate/extract
from normoxia
F. Immunoprecipitation of HIF-1 alpha protein by 2.5 μg of HIF-1
alpha antibody (GTX628480)/HepG2 whole cell lysate/extract
from hypoxia 24hr (1%O2)
10% SDS-PAGE
The immunoprecipitated HIF-1 alpha protein was detected by HIF-1 alpha antibody (GTX628480) diluted at 1:500.
EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.

REFERENCE
REVIEW

HIF1 alpha antibody [GT10211] Cat No. GTX628480

Rating ( Average 5 based on 3 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 2 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 29-Jan-2016
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : A549 cells
Fixation : 4% PFA, 2Hr
Permeabilization /Antigen retrieval : Citrate buffer, 95°C, 20minHr
Primary Antibody : 1:300, 25°C, 0.5Hr
Figure Note : A: A549 cells, B: A549 cells (1mM DMOG treatment for 3.5 h)
Date : Anonymous submitted on 29-Jan-2016
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : brain tissue
Fixation : 4% PFA, 2Hr
Permeabilization /Antigen retrieval : Citrate buffer, 95°C, 20minHr
Primary Antibody : 1:300, 25°C, 0.5Hr
Figure Note : A:Human glioblastoma tissue, B:Human healthy brain tissue
Date : Anonymous submitted on 19-Oct-2012
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : HeLa cells
Fixation : 4% paraformaldehyde
Primary Antibody : 1:250, 4°C, overnightHr
Figure Note : Right : Hypoxia (500uM CoCl2) treated 24hr, Left : untreated HeLa cells, Red: GTX628480, Green: Alpha-tubulin
SDS
PBS.pdf