GeneTex
United States (US)

HIF1 beta antibody

Orthogonal
GTX128795_41493_WB_18121410_858.jpg
GTX128795_41493_IHC_M_18121410_924.jpg
GTX128795_41493_20150410_IFA_18121410_461.jpg
GTX128795_41493_WB_Hypoxia_18121410_472.jpg

Cat No. GTX128795

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 (untreated) , HepG2 ( 1% O2 treatment for 24hr)

Predict Reactivity

Rat, Rabbit, Sheep, Bovine, Guinea pig, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human HIF1 beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HIF-1-beta Antibody, ARNT Antibody, HIF1-beta Antibody, bHLHe2 Antibody, aryl hydrocarbon receptor nuclear translocator Antibody, HIF-1beta Antibody, HIF1BETA Antibody, HIF1B Antibody, TANGO Antibody

Cellular Localization

Nucleus

Background

The aryl hydrocarbon (Ah) receptor is involved in the induction of several enzymes that participate in xenobiotic metabolism. The ligand-free, cytosolic form of the Ah receptor is complexed to heat shock protein 90. Binding of ligand, which includes dioxin and polycyclic aromatic hydrocarbons, results in translocation of the ligand-binding subunit only to the nucleus. Induction of enzymes involved in xenobiotic metabolism occurs through binding of the ligand-bound Ah receptor to xenobiotic responsive elements in the promoters of genes for these enzymes. This gene encodes a protein that forms a complex with the ligand-bound Ah receptor, and is required for receptor function. The encoded protein has also been identified as the beta subunit of a heterodimeric transcription factor, hypoxia-inducible factor 1 (HIF1). A t(1;12)(q21;p13) translocation, which results in a TEL-ARNT fusion protein, is associated with acute myeloblastic leukemia. Three alternatively spliced variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX128795_41493_WB_18121410_858.jpg

GTX128795 WB Image

HIF1 beta antibody detects HIF1 beta protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
7.5 % SDS-PAGE
HIF1 beta antibody (GTX128795) dilution: 1:1000

GTX128795_41493_IHC_M_18121410_924.jpg

GTX128795 IHC-P Image

HIF1 beta antibody detects HIF1 beta protein at nucleus on mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
HIF1 beta antibody (GTX128795) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX128795_41493_20150410_IFA_18121410_461.jpg

GTX128795 ICC/IF Image

HIF1 beta antibody detects HIF1 beta protein at nucleus by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 500μM CoCl2 for 24hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HIF1 beta protein stained by HIF1 beta antibody (GTX128795) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

GTX128795_41493_WB_Hypoxia_18121410_472.jpg

GTX128795 WB Image

Orthogonal

HIF1 beta antibody detects HIF1 beta protein by western blot analysis.
A. 30 μg HepG2 whole cell lysate/extract (untreated)
B. 30 μg HepG2 whole cell lysate/extract ( 1% O2 treatment for 24 hr)
7.5 % SDS-PAGE
HIF1 beta antibody (GTX128795) dilution: 1:1000

REVIEW

HIF1 beta antibody Cat No. GTX128795

Rating ( Average 4 based on 2 users reviews)
Application
Western Blot(WB) ( Average 4 based on 2 users reviews)
Date : Anonymous submitted on 08-Sep-2015
Score :
Application Tested : WB
Sample Species : Ms
Sample : skin fibroblast tissue
Amount used : 5μg
Blocking : 1% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Date : Anonymous submitted on 19-Aug-2015
Score :
Application Tested : WB
Sample Species : Ms
Sample : B16 melanoma cells
Amount used : 20μg
Blocking : 5% skim milk in PBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Lane Description : HIF1 beta antibody detects HIF1 beta protein by western blot analysis. Un-treated (-) and treated (+, 1% O2 treatment ) B16 melanoma cell extracts (20 μg) were separated by SDS-PAGE.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf