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Human HL-60 cells were cultured in Iscove's modified Dulbecco's medium with 4 mM L-glutamine and harvested at the log phase of growth. The HL-60 cells were fixed in 4% paraformaldehyde and arrayed on a 12-well (5 mm) adhesive coated slide, with each wellis surface specifically treated to enhance cellular attachment and to minimize background staining. Each well was loaded with approximately 7.5 x 103 HL-60 cells. A follow-up heat dehydration process was done to ensure the attachment of HL-60 cells and the stability of cellular proteins.
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