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HMBS antibody [N2C3]

Anti-HMBS antibody [N2C3] used in Western Blot (WB). GTX113460
Anti-HMBS antibody [N2C3] used in Western Blot (WB). GTX113460

Cat. No. GTX113460

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, WB-Ag

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all HMBS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
WB-Ag Assay dependent
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

K562 , human RBC

Predict Reactivity

Rat, Bovine, Cat, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HMBS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617763

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hydroxymethylbilane synthase , PBG-D , PBGD , PORC , UPS

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the hydroxymethylbilane synthase superfamily. The encoded protein is the third enzyme of the heme biosynthetic pathway and catalyzes the head to tail condensation of four porphobilinogen molecules into the linear hydroxymethylbilane. Mutations in this gene are associated with the autosomal dominant disease acute intermittent porphyria. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HMBS antibody [N2C3] used in Western Blot (WB). GTX113460

GTX113460 WB Image

Human RBC (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with HMBS antibody [N2C3] (GTX113460) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-HMBS antibody [N2C3] used in Western Blot (WB). GTX113460

GTX113460 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with HMBS antibody [N2C3] (GTX113460) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Cui X et al. Dis Model Mech 2022; (Epub) Exercise suppresses mouse systemic AApoAII amyloidosis through enhancement of the p38 MAPK signaling pathway.
Application : WB
Reactivity : Mouse
Zhu XG et al. Cell Metab 2021; 33 (1) : 211-221.e6 Functional Genomics In?Vivo Reveal Metabolic Dependencies of Pancreatic Cancer Cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169