APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500 - 1:2000 |
1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human HSPA8 (NP_006588.1).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
heat shock protein family A (Hsp70) member 8 , HEL-33 , HEL-S-72p , HSC54 , HSC70 , HSC71 , HSP71 , HSP73 , HSPA10 , LAP-1 , LAP1 , NIP71
Cellular Localization
Cytoplasm,Cell membrane,Nucleolus
Background
This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
Database
Research Area
DATA IMAGES
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GTX32660 WB Image
WB analysis of various sample lysates using GTX32660 HSC70 antibody. Dilution : 1:1000 Loading : 25μg per lane
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GTX32660 ICC/IF Image
ICC/IF analysis of L929 cells using GTX32660 HSC70 antibody. Blue : DAPI Dilution : 1:100
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REFERENCE
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REVIEW
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