HSF4 antibody [2E7]

Cat. No. GTX60581

Host	Mouse
Clonality	Monoclonal
Clone Name	2E7
Isotype	IgG1
Application	WB, FACS, ELISA
Reactivity	Human
Package	100 μg ($409)

See all HSF4 products

Datasheet
Document

View product citations for antibody GTX60581 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1/500 - 1/2000
FACS	1/200 - 1/400
ELISA	1/10000

Not tested in other applications.

Calculated MW

53 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human HSF4 expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock transcription factor 4 , CTM , CTRCT5

Cellular Localization

Nucleus

Background

Heat-shock transcription factors (HSFs) activate heat-shock response genes under conditions of heat or other stresses. HSF4 lacks the carboxyl-terminal hydrophobic repeat which is shared among all vertebrate HSFs and has been suggested to be involved in the negative regulation of DNA binding activity. Two alternatively spliced transcripts encoding distinct isoforms and possessing different transcriptional activity have been described. [provided by RefSeq, Jul 2008]

Database

Gene ID: 3299 HSF4
UniProt: Q9ULV5 HSF4

Research Area

DATA IMAGES

GTX60581 ELISA Image

ELISA analysis of antigen using GTX60581 HSF4 antibody [2E7].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

GTX60581 WB Image

WB analysis of human HSF4 (AA: 245-411) recombinant protein using GTX60581 HSF4 antibody [2E7].

GTX60581 FACS Image

FACS analysis of HeLa cells using GTX60581 HSF4 antibody [2E7].
Green : HSF4
Red : negative control

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 409

Qty