GeneTex
United States (US)

HSPA1A antibody

GTX104126_39974_IP_19011506_324.jpg
GTX104126_43138_20180406_WB_19011506_395.jpg
GTX104126_39974_IFA_19011506_532.jpg
GTX104126_39974_WB_M_brain_19011506_602.jpg
GTX104126_39974_IHC_19011506_188.jpg
GTX104126_39974_20160120_IHC-P_R_19011506_473.jpg
GTX104126_42599_20160901_WB_R_brain_19011506_172.jpg

Cat No. GTX104126

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:30000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:500-1:1000
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Mouse brain , rat brain

Predict Reactivity

Sheep, Bovine, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.67mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HSP70 1A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Heat Shock Protein Family A (Hsp70) Member 1A,Hel-S-103,Hsp70-1,Hsp70-1A,Hsp70.1,Hsp70I,Hsp72,Hspa1,Hspa1A

Background

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX104126_39974_IP_19011506_324.jpg

GTX104126 IP Image

HSP70 1A antibody immunoprecipitates HSP70 1A protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of HSP70 1A protein by 2.5 μg of HSP70 1A antibody (GTX104126) 12% SDS-PAGE The immunoprecipitated HSP70 1A protein was detected by HSP70 1A antibody (GTX104126) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX104126_43138_20180406_WB_19011506_395.jpg

GTX104126 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSPA1A antibody (GTX104126) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104126_39974_IFA_19011506_532.jpg

GTX104126 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using HSP70 1A(GTX104126) antibody at 1:200 dilution.

GTX104126_39974_WB_M_brain_19011506_602.jpg

GTX104126 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX104126 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104126_39974_IHC_19011506_188.jpg

GTX104126 IHC-P Image

HSP70 1A antibody detects HSP70 1A protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
HSP70 1A antibody (GTX104126) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104126_39974_20160120_IHC-P_R_19011506_473.jpg

GTX104126 IHC-P Image

HSP70 1A antibody detects HSP70 1A protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
HSP70 1A antibody (GTX104126) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX104126_42599_20160901_WB_R_brain_19011506_172.jpg

GTX104126 WB Image

Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSPA1A antibody (GTX104126) diluted at 1:1000.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169