APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , U87-MG , SK-N-SH , IMR32 , SK-N-AS
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.38 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human Hamartin / TSC1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886167
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
TSC complex subunit 1 , LAM , TSC
Cellular Localization
Cytoplasm , Membrane; Peripheral membrane protein
Background
This gene is a tumor suppressor gene that encodes the growth inhibitory protein hamartin. The encoded protein interacts with and stabilizes the GTPase activating protein tuberin. This hamartin-tuberin complex negatively regulates mammalian target of rapamycin complex 1 (mTORC1) signalling which is a major regulator of anabolic cell growth. This protein also functions as a co-chaperone for Hsp90 that inhibits its ATPase activity. This protein functions as a facilitator of Hsp90-mediated folding of kinase and non-kinase clients, including Tsc2 and thereby preventing their ubiquitination and proteasomal degradation. Mutations in this gene have been associated with tuberous sclerosis. [provided by RefSeq, Apr 2018]
Database
Research Area
DATA IMAGES
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GTX130062 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TSC1 antibody (GTX130062) diluted at 1:1000.
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GTX130062 IHC-P Image
TSC1 antibody detects TSC1 protein at cytoplasm in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
TSC1 antibody (GTX130062) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX130062 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TSC1 antibody (GTX130062) diluted at 1:1000.
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REFERENCE
There are currently no references for Hamartin / TSC1 antibody (GTX130062). Be the first to share your publications with this product.
REVIEW
Hamartin / TSC1 antibody
Cat. No. GTX130062
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Rating
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( Average 5 based on 2 users reviews)
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Application
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Western Blot(WB)
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( Average 5 based on 1 users reviews)
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Immunoprecipitation(IP)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 06-Jun-2022
Application Tested :
IP
Sample Species :
Hu
Sample :
HAP1 WT and TSC1 KO cells
Lane Description :
SM: 10% starting material
UB: 10% unbound fraction
IP: IP antibody
Figure Note :
Antibody-bead conjugates were prepared by adding 2 μg to 500 ul of IP Lysis Buffer, together with with 30μl of Dynabeads protein A. One ml aliquots at 1.0 mg/ml of lysate were incubated with an antibody-bead conjugate for ~2 hrs at 4ºC.
Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Date :
Anonymous submitted on 06-Jun-2022
Application Tested :
WB
Sample Species :
Hu
Sample :
HAP1 WT and TSC1 KO cells
Amount used :
100μg
Blocking :
5% milk, 1Hr
Primary Antibody :
1:500, 4°C, > 12Hr
Figure Note :
Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
