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Histone H3 (K36M Mutant Specific) antibody [RM193]

Anti-Histone H3 (K36M Mutant Specific) antibody [RM193] used in Immunohistochemistry (IHC). GTX33596
Anti-Histone H3 (K36M Mutant Specific) antibody [RM193] used in ELISA (ELISA). GTX33596
Anti-Histone H3 (K36M Mutant Specific) antibody [RM193] used in Western Blot (WB). GTX33596

Cat No. GTX33596

Host

Rabbit

Clonality

Monoclonal

Clone Name

RM193

Isotype

IgG

Application

WB, ICC/IF, ELISA, IHC

Reactivity

Human
Package
50 μg ($369)

APPLICATION

Application Note

Western Blot: 0.5 ug/mL – 2 ug/mL, ELISA: 0.5 ug/mL - 2 ug/mL, Immunohistochemistry (IHC): 0.5 – 5 ug/mL, Immunocytochemistry (ICC): 0.5 – 5 ug/mL.

Specificity/Sensitivity

This antibody reacts to the Histone H3 K36M mutant. No cross reactivity with wild type Histone H3.

PROPERTIES

Form

Liquid

Buffer

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

A peptide corresponding to Histone H3 K36M mutant

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-Histone H3 (K36M Mutant Specific) antibody [RM193] used in Immunohistochemistry (IHC). GTX33596

GTX33596 IHC Image

293T cells overexpressing Histone H3 K36M mutant stained with anti-Histone H3 K36M clone RM193.

Anti-Histone H3 (K36M Mutant Specific) antibody [RM193] used in ELISA (ELISA). GTX33596

GTX33596 ELISA Image

Sandwich ELISA performed on the lysates of 293T cells overexpressing Histone H3.3 K36M mutant or Histone H3.3 wild type His-tag proteins, shows the specificity of RM193 to the K36M mutant. His-tag fusion proteins were captured by an Anti-His-Tag antibody.

Anti-Histone H3 (K36M Mutant Specific) antibody [RM193] used in Western Blot (WB). GTX33596

GTX33596 WB Image

Western Blot analysis of cell lysates prepared from 293T overexpressing the wild type or K36M mutant proteins of Histone H3.1 and H3.3.

REFERENCE

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REVIEW

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Package List Price ($)
$ 369