GeneTex
United States (US)

Histone H3 (K36M Mutant Specific) antibody [RM193]

GTX33596_20160517_IHC_18121410_111.jpg
GTX33596_20160517_ELISA_18121410_514.jpg
GTX33596_20160517_WB_18121410_710.jpg

Cat No. GTX33596

Host Rabbit
Clonality Monoclonal
Clone Name RM193
Isotype IgG
Application WB, ICC/IF, ELISA, IHC
Reactivity Human
APPLICATION

Application Note

Western Blot: 0.5 ug/mL – 2 ug/mL, ELISA: 0.5 ug/mL - 2 ug/mL, Immunohistochemistry (IHC): 0.5 – 5 ug/mL, Immunocytochemistry (ICC): 0.5 – 5 ug/mL.

Specificity/Sensitivity

This antibody reacts to the Histone H3 K36M mutant. No cross reactivity with wild type Histone H3.
PROPERTIES

Form

Liquid

Buffer

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

A peptide corresponding to Histone H3 K36M mutant

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
GTX33596_20160517_IHC_18121410_111.jpg

GTX33596 IHC Image

293T cells overexpressing Histone H3 K36M mutant stained with anti-Histone H3 K36M clone RM193.

GTX33596_20160517_ELISA_18121410_514.jpg

GTX33596 ELISA Image

Sandwich ELISA performed on the lysates of 293T cells overexpressing Histone H3.3 K36M mutant or Histone H3.3 wild type His-tag proteins, shows the specificity of RM193 to the K36M mutant. His-tag fusion proteins were captured by an Anti-His-Tag antibody.

GTX33596_20160517_WB_18121410_710.jpg

GTX33596 WB Image

Western Blot analysis of cell lysates prepared from 293T overexpressing the wild type or K36M mutant proteins of Histone H3.1 and H3.3.

Package List Price ($)
$ 369