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Histone H3K9me3 (Tri-methyl Lys9) antibody

Orthogonal
Anti-Histone H3K9me3 (Tri-methyl Lys9) antibody used in Western Blot (WB). GTX121677
Anti-Histone H3K9me3 (Tri-methyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX121677
Anti-Histone H3K9me3 (Tri-methyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX121677
Anti-Histone H3K9me3 (Tri-methyl Lys9) antibody used in ChIP assay (ChIP assay). GTX121677
Anti-Histone H3K9me3 (Tri-methyl Lys9) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121677
Anti-Histone H3K9me3 (Tri-methyl Lys9) antibody used in Immunoprecipitation (IP). GTX121677

Cat. No. GTX121677

Reference 8
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay, EM

Reactivity

 Human, Mouse, Rat, Dog
Package
100 μl ($399),
25 μl ($169)
See all Histone H3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:1000
ChIP assay Assay dependent
EM Assay dependent
Not tested in other applications.

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding tri-methyl Lys9 of human Histone H3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10721938

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histone H3K9me3 (Tri-methyl Lys9) antibody used in Western Blot (WB). GTX121677

GTX121677 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K9me3 (Tri-methyl Lys9) antibody (GTX121677) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Histone H3K9me3 (Tri-methyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX121677

GTX121677 IHC-P Image

Histone H3K9me3 (trimethyl Lys9) antibody detects Histone H3K9me3 (trimethyl Lys9) protein at nucleus in rat kidney by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
Histone H3K9me3 (trimethyl Lys9) antibody (GTX121677) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H3K9me3 (Tri-methyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX121677

GTX121677 IHC-P Image

Histone H3K9me3 (trimethyl Lys9) antibody detects Histone H3K9me3 (trimethyl Lys9) protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Histone H3K9me3 (trimethyl Lys9) antibody (GTX121677) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H3K9me3 (Tri-methyl Lys9) antibody used in ChIP assay (ChIP assay). GTX121677

GTX121677 ChIP assay Image

Cross-linked ChIP was performed with HepG2 chromatin extract and 5 μg of either normal rabbit IgG or anti-Histone H3K9me3 (trimethyl Lys9) antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.

Anti-Histone H3K9me3 (Tri-methyl Lys9) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121677

GTX121677 ICC/IF Image

Histone H3K9me3 (trimethyl Lys9) antibody detects Histone H3K9me3 (trimethyl Lys9) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H3K9me3 (trimethyl Lys9) protein stained by Histone H3K9me3 (trimethyl Lys9) antibody (GTX121677) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Histone H3K9me3 (Tri-methyl Lys9) antibody used in Immunoprecipitation (IP). GTX121677

GTX121677 IP Image

Immunoprecipitation of Histone H3 (tri-Methyl Lys9) protein from 293T whole cell extracts using 5 μg of Histone H3 (tri-Methyl Lys9) (GTX121677).
Western blot analysis was performed using Histone H3 (tri-Methyl Lys9) (GTX121677) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE

Application Reference

C Casali et al. Eur J Cell Biol 2023; 103 (1) : 151373 Impact of heat and cold shock on epigenetics and chromatin structure
Application : EM
He Y et al. Clin Epigenetics 2022; 14 (1) : 101 JIB-04, a histone demethylase Jumonji C domain inhibitor, regulates phenotypic switching of vascular smooth muscle cells.
Application : WB, IHC-P
Reactivity : Human
Xin Yi et al. Front Cardiovasc Med 2021; 8 : 692856 The Expression Patterns and Roles of Lysyl Oxidases in Aortic Dissection.
Application : WB
Reactivity : Human
Hern?ndez-Guzm?n C et al. Cells 2021; 10 (10) Zonula occludens 2 and Cell-Cell Contacts Are Required for Normal Nuclear Shape in Epithelia.
Application : ICC/IF
Reactivity : Dog
Jia Z Shen et al. Cell 2020; S0092-8674 (20) : 31614-7 FBXO44 promotes DNA replication-coupled repetitive element silencing in cancer cells.
Application : WB
Reactivity : Human
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Submit a Reference

REVIEW

Histone H3K9me3 (Tri-methyl Lys9) antibody Cat. No. GTX121677

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Electron microscopy(EM)
( Average 5 based on 1 users reviews)
Anti-Histone H3K9me3 (Tri-methyl Lys9) antibody used in Western Blot (WB). GTX121677
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : MG63 cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:100, 4°C, 16Hr
Lane Description : Lane 1: MG63 whole cell lysate, Lane 2: doxorubicin resistant MG63 cell line, Lane 3: Cisplatin resistant MG63 cell line
Anti-Histone H3K9me3 (Tri-methyl Lys9) antibody used in Electron microscopy (EM). GTX121677
Date : Anonymous submitted on 27-Mar-2017
Score :
Application Tested : EM
Sample Species : Ms
Sample : liver tissue
Blocking : Normal Goat Serum in PBS, 25°C, 0.08Hr
Fixation : 4% PFA in S?rensen phosphate buffer (pH 7.2) or in culture medium
Permeabilization /Antigen retrieval : 0.1% BSA in PBST
Primary Antibody : 1:10, 4°C, 16Hr
Submit a Review
Application Reference
C Casali et al. Eur J Cell Biol 2023; 103 (1):151373 Impact of heat and cold shock on epigenetics and chromatin structure
Application : EM
He Y et al. Clin Epigenetics 2022; 14 (1):101 JIB-04, a histone demethylase Jumonji C domain inhibitor, regulates phenotypic switching of vascular smooth muscle cells.
Application : WB, IHC-P
Reactivity : Human
Xin Yi et al. Front Cardiovasc Med 2021; 8:692856 The Expression Patterns and Roles of Lysyl Oxidases in Aortic Dissection.
Application : WB
Reactivity : Human
Hern?ndez-Guzm?n C et al. Cells 2021; 10 (10) Zonula occludens 2 and Cell-Cell Contacts Are Required for Normal Nuclear Shape in Epithelia.
Application : ICC/IF
Reactivity : Dog
Jia Z Shen et al. Cell 2020; S0092-8674 (20):31614-7 FBXO44 promotes DNA replication-coupled repetitive element silencing in cancer cells.
Application : WB
Reactivity : Human
Kim SY et al. Chem Biol Interact 2020; 332:109277 Molecular mechanisms underlying the effects of the small molecule AMC-04 on apoptosis: Roles of the activating transcription factor 4-C/EBP homologous protein-death receptor 5 pathway.
Application : WB
Reactivity : Human
Tateishi-Karimata H et al. J Am Chem Soc 2018; 140 (2):642-651 Destabilization of DNA G-Quadruplexes by Chemical Environment Changes during Tumor Progression Facilitates Transcription.
Application : ICC/IF
Reactivity : Human
Li KC et al. Mol Cancer 2014; 13:172 Inhibition of G9a induces DUSP4-dependent autophagic cell death in head and neck squamous cell carcinoma.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169