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HuC antibody

Anti-HuC antibody used in IHC (Paraffin sections) (IHC-P). GTX134128
Anti-HuC antibody used in IHC (Frozen sections) (IHC-Fr). GTX134128
Anti-HuC antibody used in IHC (Paraffin sections) (IHC-P). GTX134128
Anti-HuC antibody used in IHC (Paraffin sections) (IHC-P). GTX134128
Anti-HuC antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134128
Anti-HuC antibody used in Western Blot (WB). GTX134128
Anti-HuC antibody used in IHC (Frozen sections) (IHC-Fr). GTX134128

Cat No. GTX134128

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Human(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.26 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse HuC. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ELAV like RNA binding protein 3,2600009P04Rik,Huc,PLE21,mHuC

Background

Binds to AU-rich sequences (AREs) of target mRNAs, including VEGF mRNA. May also bind poly-A tracts via RRM 3. May be involved in neuronal differentiation and maintenance.

Database

DATA IMAGES
Anti-HuC antibody used in IHC (Paraffin sections) (IHC-P). GTX134128

GTX134128 IHC-P Image

HuC antibody detects HuC protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
HuC stained by HuC antibody (GTX134128) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-HuC antibody used in IHC (Frozen sections) (IHC-Fr). GTX134128

GTX134128 IHC-Fr Image

HuC antibody detects HuC protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse hippocampus.
Green: HuC stained by HuC antibody (GTX134128) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-HuC antibody used in IHC (Paraffin sections) (IHC-P). GTX134128

GTX134128 IHC-P Image

HuC antibody detects HuC protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
HuC stained by HuC antibody (GTX134128) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-HuC antibody used in IHC (Paraffin sections) (IHC-P). GTX134128

GTX134128 IHC-P Image

HuC antibody detects HuC protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
HuC stained by HuC antibody (GTX134128) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-HuC antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134128

GTX134128 ICC/IF Image

HuC antibody detects HuC protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HuC stained by HuC antibody (GTX134128) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-HuC antibody used in Western Blot (WB). GTX134128

GTX134128 WB Image

Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with HuC antibody (GTX134128) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HuC antibody used in IHC (Frozen sections) (IHC-Fr). GTX134128

GTX134128 IHC-Fr Image

HuC antibody detects HuC protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse hippocampus.
Green: HuC stained by HuC antibody (GTX134128) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169