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Human BMP2 protein (active)

Human BMP2 protein (active). GTX00202-pro
Human BMP2 protein (active). GTX00202-pro
Human BMP2 protein (active). GTX00202-pro

Cat No. GTX00202-pro

Application Functional Assay
Species Human
Package
10 μg ($269)
APPLICATION

Application Note

Bone morphogenetic protein 2 (BMP2) belongs to the TGF-β superfamily of proteins. It plays an important role in the development of bone and cartilage. It is involved in the hedgehog pathway, TGF beta signaling pathway, and in cytokine-cytokine receptor interaction. BMP2 is also involved in cardiac cell differentiation and epithelial to mesenchymal transition. Like many other proteins from the BMP family, BMP2 has been demonstrated to potently induce osteoblast differentiation in a variety of cell types. Besides, Noggin (NOG) has been identified as an interactor of BMP2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human BMP2 and recombinant human NOG. Briefly, BMP2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to NOG-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-BMP2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of BMP2 and NOG was in a dose dependent manner.

Observed MW

43 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His and GST-Tag; Gln283~Arg396 (NP_001191.1)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

bone morphogenetic protein 2,BDA2,BMP2A,SSFSC

Background

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer, which plays a role in bone and cartilage development. Duplication of a regulatory region downstream of this gene causes a form of brachydactyly characterized by a malformed index finger and second toe in human patients. [provided by RefSeq, Jul 2016]

Database

Research Area

DATA IMAGES
Human BMP2 protein (active). GTX00202-pro

GTX00202-pro Functional Assay Image

Functional ELISA analysis of GTX00202-pro Human BMP2 protein (active) which can bind immobilized NOG protein.

Human BMP2 protein (active). GTX00202-pro

GTX00202-pro Image

SDS-PAGE analysis of GTX00202-pro Human BMP2 protein (active).

Human BMP2 protein (active). GTX00202-pro

GTX00202-pro Image

WB analysis of GTX00202-pro Human BMP2 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 269