PRODUCT
Summary
Multimeric Proteins are high activity constructs in which two trimeric TNFSF ligands are linked via the oligomeric collagen domain of ACRP30 [ACRP30headless] and therefore mimic the membrane-bound forms of the proteins.
APPLICATION
Application Note
Induces B cells activation (as demonstrated by dose-dependent upregulation of CD86) (ED₅₀:: <1ng/ml).
Product Note
Binds to human CD40.
PROPERTIES
Form
Lyophilized powder
Buffer
Reconstitute with 100μl ddH₂O to 0.1mg/ml. Lyophilized from 0.2μm-filtered PBS.
Preservative
No preservative
Storage
Store at -20ºC or below. After reconstitution, keep as concentrated solution. Aliquot and avoid freeze-thaw cycles.
Region/Sequence
N-terminal DDDDK-Tag; Human CD40L (a.a.116-261) is fused at the N-terminus to mouse ACRP30headless (a.a.18-111).
Expression System
CHO cells
Purity
≥ 95% by SDS-PAGE.
Endotoxin
< 0.01 EU/μg
Conjugation
Unconjugated
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
CD40 ligand , CD154 , CD40L , HIGM1 , IGM , IMD3 , T-BAM , TNFSF5 , TRAP , gp39 , hCD40L
Database
Research Area
DATA IMAGES
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GTX65635-pro Image
GTX65635-pro CD40L protein (multimeric) does not need an enhancer to induce B cells activation. Method: PBL cells were incubated in 96-well plates (2x105 cells/well in 100μl RPMI supplemented with 10% FCS) for 24 hours at 37ºC with the indicated concentration of CD40L (multimeric) or CD40L in the presence and absence of 1μg/ml Enhancer. Cells were washed with PBS and stained with CD86-PE antibody and CD19-FITC antibody, and then analyzed by flow cytometry.
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REFERENCE
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REVIEW
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