• Country / Location Selection

United States (US)

Human FGF1 protein (active)

Human FGF1 protein (active). GTX00081-pro
Human FGF1 protein (active). GTX00081-pro
Human FGF1 protein (active). GTX00081-pro

Cat No. GTX00081-pro


Functional Assay


10 μg ($229)


Application Note

FGF1 (Fibroblast growth factor 1) is a member of FGF family, which plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. FGF1 is thought to stimulate the proliferation of 3T3 fibroblasts, besides, human FGF1 and mouse FGF1 shares similarities in amino acid sequence with the identity of 96% . Thus, a cell proliferation assay was conducted to detect the bioactivity of recombinant human FGF1 using 3T3 fibroblasts. Briefly,  3T3 cells were seeded into triplicate wells of 96-well plates at a density of 2000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM  prior to the addition of various concentrations of FGF1. After incubated for 48h,  cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37ºC. Proliferation of 3T3 cells after incubation with FGF1 for 48h observed by inverted microscope. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant FGF1 for 48h. And FGF1 significantly increased cell viability of 3T3 cells.

Observed MW

55 kDa.



Lyophilized powder


Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.


For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.


Full length protein, N-terminal His and GST-Tag; Met1~Asp155 (NP_000791.1)

Expression System

E. coli


> 97%


< 1 EU/μg




For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.



fibroblast growth factor 1 , AFGF , ECGF , ECGF-beta , ECGFA , ECGFB , FGF-1 , FGF-alpha , FGFA , GLIO703 , HBGF-1 , HBGF1


The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Jan 2009]


Research Area


Human FGF1 protein (active). GTX00081-pro

GTX00081-pro Functional Assay Image

Cell proliferation effect of GTX00081-pro Human FGF1 protein (active). Cell viability was measured by Cell Counting Kit-8 (CCK-8).
(A) Unstimulated 3T3 cells cultured in DMEM for 48hrs.
(B) 3T3 cells cultured in DMEM, stimulated with 100 ng/ml FGF1 for 48hrs.

Human FGF1 protein (active). GTX00081-pro

GTX00081-pro Image

WB analysis of GTX00081-pro Human FGF1 protein (active).

Human FGF1 protein (active). GTX00081-pro

GTX00081-pro Image

SDS-PAGE analysis of GTX00081-pro Human FGF1 protein (active).


There are currently no references for Human FGF1 protein (active) (GTX00081-pro). Be the first to share your publications with this product.


There are currently no reviews for Human FGF1 protein (active) (GTX00081-pro). Be the first to share your experience with this product.
Package List Price ($)
$ 229