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Human FGF1 protein (active)

Human FGF1 protein (active). GTX00081-pro
Human FGF1 protein (active). GTX00081-pro
Human FGF1 protein (active). GTX00081-pro

Cat No. GTX00081-pro

Application Functional Assay
Species Human
Package
10 μg ($229)
APPLICATION

Application Note

FGF1 (Fibroblast growth factor 1) is a member of FGF family, which plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. FGF1 is thought to stimulate the proliferation of 3T3 fibroblasts, besides, human FGF1 and mouse FGF1 shares similarities in amino acid sequence with the identity of 96% . Thus, a cell proliferation assay was conducted to detect the bioactivity of recombinant human FGF1 using 3T3 fibroblasts. Briefly,  3T3 cells were seeded into triplicate wells of 96-well plates at a density of 2000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM  prior to the addition of various concentrations of FGF1. After incubated for 48h,  cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37ºC. Proliferation of 3T3 cells after incubation with FGF1 for 48h observed by inverted microscope. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant FGF1 for 48h. And FGF1 significantly increased cell viability of 3T3 cells.

Observed MW

55 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

Full length protein, N-terminal His and GST-Tag; Met1~Asp155 (NP_000791.1)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

fibroblast growth factor 1 , AFGF , ECGF , ECGF-beta , ECGFA , ECGFB , FGF-1 , FGF-alpha , FGFA , GLIO703 , HBGF-1 , HBGF1

Background

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Jan 2009]

Database

Research Area

DATA IMAGES
Human FGF1 protein (active). GTX00081-pro

GTX00081-pro Functional Assay Image

Cell proliferation effect of GTX00081-pro Human FGF1 protein (active). Cell viability was measured by Cell Counting Kit-8 (CCK-8).
(A) Unstimulated 3T3 cells cultured in DMEM for 48hrs.
(B) 3T3 cells cultured in DMEM, stimulated with 100 ng/ml FGF1 for 48hrs.

Human FGF1 protein (active). GTX00081-pro

GTX00081-pro Image

WB analysis of GTX00081-pro Human FGF1 protein (active).

Human FGF1 protein (active). GTX00081-pro

GTX00081-pro Image

SDS-PAGE analysis of GTX00081-pro Human FGF1 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 229