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Human Hepcidin protein (active)

Human Hepcidin protein (active). GTX00261-pro
Human Hepcidin protein (active). GTX00261-pro
Human Hepcidin protein (active). GTX00261-pro

Cat No. GTX00261-pro

Application Functional Assay
Species Human
Package
10 μg ($229)
APPLICATION

Application Note

Hepcidin (Hepc) is a regulator of iron metabolism. Hepcidin inhibits iron transport by binding to the iron export channel ferroportin which is located on the basolateral surface of gut enterocytes and the plasma membrane of reticuloendothelial cells (macrophages). Hepcidin ultimately breaks down the transporter protein in the lysosome. Inhibiting ferroportin prevents iron from being exported and the iron is sequestered in the cells. Besides, Ferroportin (FPN) has been identified as an interactor of Hepc, thus a binding ELISA assay was conducted to detect the interaction of recombinant human Hepc and recombinant human FPN. Briefly, Hepc were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to FPN-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-Hepc pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of Hepc and FPN was in a dose dependent manner.

Observed MW

34 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from PBS (pH 7.4) with 0.01% SKL, 1 mM DTT, 5% Trehalose, Proclin300. Reconstitute with 10 mM PBS (pH 7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ser25~Thr84 (NP_066998.1)

Expression System

293F cell

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

hepcidin antimicrobial peptide,HEPC,HFE2B,LEAP1,PLTR

Background

The product encoded by this gene is involved in the maintenance of iron homeostasis, and it is necessary for the regulation of iron storage in macrophages, and for intestinal iron absorption. The preproprotein is post-translationally cleaved into mature peptides of 20, 22 and 25 amino acids, and these active peptides are rich in cysteines, which form intramolecular bonds that stabilize their beta-sheet structures. These peptides exhibit antimicrobial activity against bacteria and fungi. Mutations in this gene cause hemochromatosis type 2B, also known as juvenile hemochromatosis, a disease caused by severe iron overload that results in cardiomyopathy, cirrhosis, and endocrine failure. [provided by RefSeq, Oct 2014]

Database

DATA IMAGES
Human Hepcidin protein (active). GTX00261-pro

GTX00261-pro Functional Assay Image

Functional ELISA analysis of GTX00261-pro Human Hepcidin protein (active) which can bind immobilized FPN protein.

Human Hepcidin protein (active). GTX00261-pro

GTX00261-pro Image

SDS-PAGE analysis of GTX00261-pro Human Hepcidin protein (active).

Human Hepcidin protein (active). GTX00261-pro

GTX00261-pro Image

WB analysis of GTX00261-pro Human Hepcidin protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 229