APPLICATION
Application Note
Inhibits adipogenesis of mesenchymal stem cells (MSCs). Induces Hes-1 in 3T3L-1 cells.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
Concentration
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Region/Sequence
Signal peptide and extracellular domain of human Jagged-1 (HJ1; CD339) (a.a. 1-1067) are fused at the C-terminus to the Fc portion of human IgG1.
Expression System
HEK293 cells
Purity
≥ 90% by SDS-PAGE.
Endotoxin
< 0.1 EU/μg
Conjugation
Unconjugated
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
jagged canonical Notch ligand 1 , AGS , AGS1 , AHD , AWS , CD339 , DCHE , HJ1 , JAGL1
Database
Research Area
DATA IMAGES
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GTX65636-pro Image
Induction of Hes-1 with the treatment of GTX65636-pro Jagged 1 Fc fusion protein. A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5μg/ml of human Jagged1-Fc fusion protein as in indicated time points and each cell lysate was prepared and subjected to western blot by using human Hes1 antibody or GAPDH antibody.
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GTX65636-pro Image
Adipogenesis inhibition of 3T3L-1 cells. When the cells were confluently grown adipogenesis was initiated by adding IBMX, Dexamethasone, and insulin to 0.5mM, 1μM, and 10μg/ml, respectively and continued for 2 days (day 0).The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5μg/ml of human Jagged1-Fc (GTX65636-pro) and human CD137-Fc as a control-Fc. Staining with Oil Red O was typically performed on day 7.
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REFERENCE
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REVIEW
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