Form
Liquid
Buffer
20mM Tris-HCl buffer (pH8.0), 0.2M NaCl, 2mM DTT, 10% Glycerol
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Region/Sequence
Full length protein, N-terminal His-Tag; MGSSHHHHHH SSGLVPRGSH MGSHMLRPKA LTQVLSQANT GGVQSTLLLN NEGSLLAYSG YGDTDARVTA AIASNIWAAY DRNGNQAFNE DNLKFILMDC MEGRVAITRV ANLLLCMYAK ETVGFGMLKA KAQALVQYLE EPLTQVAAS
Expression System
E. coli
Purity
> 95% by SDS-PAGE.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
late endosomal/lysosomal adaptor, MAPK and MTOR activator 2 , ENDAP , HSPC003 , MAPBPIP , MAPKSP1AP , ROBLD3 , Ragulator2 , p14
Cellular Localization
Lysosome membrane
Background
The product of this gene is highly conserved with a mouse protein associated with the cytoplasmic face of late endosomes and lysosomes. The mouse protein interacts with MAPK scaffold protein 1, a component of the mitogen-activated protein kinase pathway. In humans, a mutation in this gene has been associated with a primary immunodeficiency syndrome, and suggests a role for this protein in endosomal biogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]
Database
Research Area