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Human Lactoferrin protein (active)

Human Lactoferrin protein (active). GTX00220-pro
Human Lactoferrin protein (active). GTX00220-pro
Human Lactoferrin protein (active). GTX00220-pro

Cat No. GTX00220-pro

Application

Functional Assay

Species

Human
Package
10 μg ($269)

APPLICATION

Application Note

Lactoferrin (LF), also known as lactotransferrin (LTF), is a multifunctional protein of the transferrin family. Lactoferrin is one of the components of the immune system of the body; it has antimicrobial activity (bacteriocide, fungicide) and is part of the innate defense, mainly at mucoses. In particular, lactoferrin provides antibacterial activity to human infants. Lactoferrin interacts with DNA and RNA, polysaccharides and heparin, and shows some of its biological functions in complexes with these ligands. LTF is known to activate immune cells to produce several cytokines, such as tumor necrosis factor TNF-a, thus, a stimulation assay was conducted to detect the activity of LTF using human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into wells of 24-well plates at a density of 5x10⁶ cells/ml in RPMI-1640 with the addition of various concentrations of recombinant human LTF. After incubation for 20 hours, the concentration of TNF-a in the cell supernatant was detected using an ELISA kit. TNF-a levels in the cell supernatant of spleen cells increased significantly after stimulated with LTF.

Observed MW

80 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from PBS (pH 7.4) with 0.01% SKL, 1 mM DTT, 5% Trehalose, Proclin300. Reconstitute with 10 mM PBS (pH 7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Native Protein (NP_001186078.1)

Expression System

native protein from human (Breast)

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

lactotransferrin , GIG12 , HEL110 , HLF2 , LF

Background

This gene is a member of the transferrin family of genes and its protein product is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. Antimicrobial, antiviral, antifungal and antiparasitic activity has been found for this protein and its peptides. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

Database

Research Area

DATA IMAGES

Human Lactoferrin protein (active). GTX00220-pro

GTX00220-pro Functional Assay Image

ELISA detection of secreted TNF alpha protein activation from THP-1 cells induced by GTX00220-pro Human Lactoferrin protein (active) for 20hrs.
Recombinant protein concentrations: 10, 100, and 1000 ng/ml.

Human Lactoferrin protein (active). GTX00220-pro

GTX00220-pro Image

WB analysis of GTX00220-pro Human Lactoferrin protein (active).

Human Lactoferrin protein (active). GTX00220-pro

GTX00220-pro Image

SDS-PAGE analysis of GTX00220-pro Human Lactoferrin protein (active).

REFERENCE

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REVIEW

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Package List Price ($)
$ 269