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Human Prokineticin 2 protein (active)

Human Prokineticin 2 protein (active). GTX00253-pro
Human Prokineticin 2 protein (active). GTX00253-pro
Human Prokineticin 2 protein (active). GTX00253-pro

Cat No. GTX00253-pro

Application Functional Assay
Species Human
Package
10 μg ($279)
APPLICATION

Application Note

Prokineticin 2 (PK2) is a memeber of prokineticin family. Prokineticin is a secreted protein that potently contracts gastrointestinal smooth muscle. They are thought to be involved in several important physiological processes like neurogenesis, tissue development, angiogenesis, and nociception. Other important physiological roles the Bv8/Prokineticins (PKs) are involved in may include cancer, reproduction, and regulating physiological functions that influence circadian rhythms like hormone secretion, ingestive behaviors, and the sleep/wake cycle. To test the effect of PK2 on cell proliferation, HCT116 colon cancer cells were seeded into triplicate wells of 96-well plates at a density of 5000 cells/well and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 2% serum standard DMEM containing various concentrations of recombinant human PK2. After incubated for 96h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37ºC. Proliferation of HCT116 cells after incubation with PK2 for 96h observed by inverted microscope.

Observed MW

39 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ile30~Gln128 (NP_001119600.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

prokineticin 2,BV8,HH4,KAL4,MIT1,PK2

Background

This gene encodes a protein expressed in the suprachiasmatic nucleus (SCN) circadian clock that may function as the output component of the circadian clock. The secreted form of the encoded protein may also serve as a chemoattractant for neuronal precursor cells in the olfactory bulb. Proteins from other vertebrates which are similar to this gene product were isolated based on homology to snake venom and secretions from frog skin, and have been shown to have diverse functions. Mutations in this gene are associated with Kallmann syndrome 4. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Human Prokineticin 2 protein (active). GTX00253-pro

GTX00253-pro Functional Assay Image

Cell proliferation effect of GTX00253-pro Human Prokineticin 2 protein (active). Cell viability was measured by Cell Counting Kit-8 (CCK-8).
(A) Unstimulated HCT116 cells cultured in DMEM for 96hrs.
(B) HCT116 cells cultured in DMEM, stimulated with 100 ng/ml PK2 for 96hrs.

Human Prokineticin 2 protein (active). GTX00253-pro

GTX00253-pro Image

SDS-PAGE analysis of GTX00253-pro Human Prokineticin 2 protein (active).

Human Prokineticin 2 protein (active). GTX00253-pro

GTX00253-pro Image

WB analysis of GTX00253-pro Human Prokineticin 2 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 279