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Human S100A6 protein, His tag

Human S100A6 protein, His tag. GTX00135-pro
Human S100A6 protein, His tag. GTX00135-pro
Human S100A6 protein, His tag. GTX00135-pro

Cat. No. GTX00135-pro

Application

 Functional Assay

Species

 Human
Package
10 μg ($309)
See all S100A6 protein products

APPLICATION

Application Note

S100A6 is a calcium-binding protein, which functions as calcium sensor and modulator, contributing to cellular calcium signaling. It is reported that S100B and S100A6 differentially modulate cell survival by Interacting with distinct RAGE (Receptor for Advanced Glycation End Products). Thus a binding ELISA assay was conducted to detect the interaction of S100A6 and RAGE. Briefly, recombinant human S100A6 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to RAGE-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-S100A6 mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of of S100A6 and RAGE was in a dose dependent manner.

Observed MW

16 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

Full length protein, N-terminal His-Tag; Met1~Gly90 (NP_055439.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

S100 calcium binding protein A6 , 2A9 , 5B10 , CABP , CACY , PRA , S10A6

Background

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-dependent insulin release, stimulation of prolactin secretion, and exocytosis. Chromosomal rearrangements and altered expression of this gene have been implicated in melanoma. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Human S100A6 protein, His tag. GTX00135-pro

GTX00135-pro Functional Assay Image

Functional ELISA analysis of GTX00135-pro Human S100A6 protein which can bind immobilized RAGE protein.

Human S100A6 protein, His tag. GTX00135-pro

GTX00135-pro Image

WB analysis of GTX00135-pro Human S100A6 protein.

Human S100A6 protein, His tag. GTX00135-pro

GTX00135-pro Image

SDS-PAGE analysis of GTX00135-pro Human S100A6 protein.

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 309