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ICAM1 / CD54 antibody [15.2] (FITC)

Anti-ICAM1 / CD54 antibody [15.2] (FITC) used in Flow cytometry (FACS). GTX75217

Cat. No. GTX75217

Reference 15

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 15.2

Isotype

 IgG1

Application

 FACS

Reactivity

 Human, Rabbit, Pig
Package
100 test ($359)
See all ICAM1 / CD54 products
PRODUCT

Summary

The 15.2 antibody reacts with human CD54, also known as ICAM-1 (Intercellular Adhesion Molecule 1), a 90-110 kDa cell surface glycoprotein that is inducibly expressed on both immune and endothelial cells. As its name implies, ICAM-1 participates in cell-cell adhesion between leukocytes and endothelial cells, facilitating leukocyte recruitment and transmigration at sites of inflammation. The ligands for ICAM-1 are also expressed on leukocyte and endothelial cells, and include Mac-1, fibrinogen, and a member of the integrin αprotein family, LFA-1 (CD11a).

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS 0.5 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human monocytes

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Fluorescein isothiocyanate (FITC)

RRID

AB_376664

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

intercellular adhesion molecule 1 , BB2 , CD54 , P3.58

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ICAM1 / CD54 antibody [15.2] (FITC) used in Flow cytometry (FACS). GTX75217

GTX75217 FACS Image

FACS analysis of human peripheral blood monocytes using GTX75217 ICAM1 / CD54 antibody [15.2] (FITC).
Solid line : Primary antibody
Dashed line : FITC mouse IgG1 isotype control
Antibody amount : 0.5 μg

REFERENCE

Application Reference

Lee TC et al. PLoS One 2014; 9 (11) : e111390 Comparison of Surface Markers between Human and Rabbit Mesenchymal Stem Cells.
Application : FACS
Reactivity : Human, Rabbit

Clone Reference

Eun-Ji Lim et al. Cell Death Dis 2022; 13 (4):417 ICAM-1 promotes cancer progression by regulating SRC activity as an adapter protein in colorectal cancer.
Chiang HY et al. J Biomed Sci 2019; 26 (1):61 MFG-E8 mediates arterial aging by promoting the proinflammatory phenotype of vascular smooth muscle cells.
Lin JC et al. Neoplasia 2019; 21 (5):459-468 Musashi-1 Enhances Glioblastoma Migration by Promoting ICAM1 Translation.
Fan JR et al. Cell Death Dis 2018; 9 (5):502 Potential role of CBX7 in regulating pluripotency of adult human pluripotent-like olfactory stem cells in stroke model.
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REVIEW

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Application Reference
Lee TC et al. PLoS One 2014; 9 (11):e111390 Comparison of Surface Markers between Human and Rabbit Mesenchymal Stem Cells.
Application : FACS
Reactivity : Human, Rabbit
Clone Reference
Eun-Ji Lim et al. Cell Death Dis 2022; 13 (4):417 ICAM-1 promotes cancer progression by regulating SRC activity as an adapter protein in colorectal cancer.
Chiang HY et al. J Biomed Sci 2019; 26 (1):61 MFG-E8 mediates arterial aging by promoting the proinflammatory phenotype of vascular smooth muscle cells.
Lin JC et al. Neoplasia 2019; 21 (5):459-468 Musashi-1 Enhances Glioblastoma Migration by Promoting ICAM1 Translation.
Fan JR et al. Cell Death Dis 2018; 9 (5):502 Potential role of CBX7 in regulating pluripotency of adult human pluripotent-like olfactory stem cells in stroke model.
2012; The transcription factor Erg controls endothelial cell quiescence by repressing activity of nuclear factor (NF)-κB p65
2012; A restricted subset of var genes mediates adherence of Plasmodium falciparum-infected erythrocytes to brain endothelial cells
2012; Multiple receptors trigger human NK cell-mediated cytotoxicity against porcine chondrocytes
2011; Endothelial reticulon-4B (Nogo-B) regulates ICAM-1-mediated leukocyte transmigration and acute inflammation
2010; Perturbation of adhesion molecule-mediated chondrocyte-matrix interactions by 4-hydroxynonenal binding: implication in osteoarthritis pathogenesis
Tran DQ et al. J Immunol 2009; 182 (5):2929-38 Analysis of adhesion molecules, target cells, and role of IL-2 in human FOXP3+ regulatory T cell suppressor function.
2009; Fibrinogen binding to ICAM-1 promotes EGFR-dependent mucin production in human airway epithelial cells
2005; Effect of long-term culture on the expression of antigens and adhesion molecule in single porcine pancreatic endocrine cells
2000; Human bronchial intraepithelial T lymphocytes as a distinct T-cell subset: their long-term survival in SCID-Hu chimeras
1997; Contribution of virion ICAM-1 to human immunodeficiency virus infectivity and sensitivity to neutralization
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 359