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ID1 antibody [195-14]

Anti-ID1 antibody [195-14] used in Western Blot (WB). GTX44599
Anti-ID1 antibody [195-14] used in Immunohistochemistry (IHC). GTX44599

Cat. No. GTX44599

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 195-14

Isotype

 IgG

Application

 WB, IHC-P, IHC

Reactivity

 Human, Mouse
Package
100 μg ($1179)
See all ID1 products

APPLICATION

Application Note

Recommended Starting Dilutions:
IHC-P: Use at 1/100 dilution and incubate for 2 hours at room temperature
WB: Dilute 1/500 in a suitable diluent and incubate for 1-16 hours at room temp
Optimal dilutions should be determined experimentally by the researcher.

Calculated MW

16 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Tissue culture supernatant, 0.25% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.05 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant full length mouse ID1 recombinant protein.

Purification

Tissue Culture Supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

inhibitor of DNA binding 1, HLH protein , ID , bHLHb24

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The encoded protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ID1 antibody [195-14] used in Western Blot (WB). GTX44599

GTX44599 WB Image

Western Blot with clone 195-14.

Lane 1: Whole cell extract from Id1- /- mouse embryonic fibroblasts.

Lane 2: Whole cell extract from Id1+/+ mouse embryonic fibroblasts.

Lane 3: Whole cell extract from HeLa cells.
(Perk et.al.)

Anti-ID1 antibody [195-14] used in Immunohistochemistry (IHC). GTX44599

GTX44599 IHC Image

IHC with clone 195-14. Invasive ductal carcinoma human triple neg. mammary tumor (negative for PR, negative for ER, and negative for Her-2/Neu) with several tumor cells (TC) expressing Id1. (Perk et. al.)

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 1179