GeneTex

  • Country / Location Selection

United States (US)

ID3 antibody [10D3]

Overexpression
Anti-ID3 antibody [10D3] used in Western Blot (WB). GTX84325
Anti-ID3 antibody [10D3] used in Flow cytometry (FACS). GTX84325
Anti-ID3 antibody [10D3] used in Flow cytometry (FACS). GTX84325
Anti-ID3 antibody [10D3] used in Western Blot (WB). GTX84325
Anti-ID3 antibody [10D3] used in IHC (Paraffin sections) (IHC-P). GTX84325

Cat. No. GTX84325

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 10D3

Isotype

 IgG1

Application

 WB, IHC-P, FACS

Reactivity

 Human, Dog
Package
100 μl ($519)
See all ID3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.61 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human ID3 (NP_002158) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

inhibitor of DNA binding 3, HLH protein , HEIR-1 , bHLHb25

Cellular Localization

Nucleus

Background

ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-3 inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. May inhibit other transcription factors.

Database

Research Area

DATA IMAGES

Anti-ID3 antibody [10D3] used in Western Blot (WB). GTX84325

GTX84325 WB Image

WB analysis of various cell lines using GTX84325 ID3 antibody [10D3].
Loading : 10 ug per lane
Dilution : 1:200

Anti-ID3 antibody [10D3] used in Flow cytometry (FACS). GTX84325

GTX84325 FACS Image

FACS analysis of HeLa cells using GTX84325 ID3 antibody [10D3].
Red : Primary antibody
Blue : Negative control antibody

Anti-ID3 antibody [10D3] used in Flow cytometry (FACS). GTX84325

GTX84325 FACS Image

FACS analysis of Jurkat cells using GTX84325 ID3 antibody [10D3].
Red : Primary antibody
Blue : Negative control antibody

Anti-ID3 antibody [10D3] used in Western Blot (WB). GTX84325

GTX84325 WB Image

Overexpression

WB analysis of HEK293T cells transfected with ID3 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84325 ID3 antibody [10D3].
Loading : 5 ug per lane

Anti-ID3 antibody [10D3] used in IHC (Paraffin sections) (IHC-P). GTX84325

GTX84325 IHC-P Image

IHC-P analysis of human liver tissue using GTX84325 ID3 antibody [10D3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

REFERENCE

There are currently no references for ID3 antibody [10D3] (GTX84325). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for ID3 antibody [10D3] (GTX84325). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519