Wild-type (WT) and IDE Kip1 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IDE antibody [N3C1], Internal (GTX111664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IDE antibody [N3C1], Internal (GTX111664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using insulin degrading enzyme(GTX111664) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

IDE antibody [N3C1], Internal detects IDE protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: IDE stained by IDE antibody [N3C1], Internal (GTX111664) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Various extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IDE antibody [N3C1], Internal (GTX111664) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

IDE antibody [N3C1], Internal detects IDE protein at cytosol on rat brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain stem.
IDE antibody [N3C1], Internal (GTX111664) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

IDE antibody [N3C1], Internal detects Ide protein at the myotome on whole-mount zebrafish embryos by immunohistochemical analysis.?
Sample: Paraformaldehyde-fixed zebrafish embryos.
IDE antibody [N3C1], Internal (GTX111664) dilution: 1:200.

The data was published in the journal PLoS One in 2018.PMID: 29715265