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IDH1 antibody [2H9]

KO/KD Overexpression
Anti-IDH1 antibody [2H9] used in Western Blot (WB). GTX84319
Anti-IDH1 antibody [2H9] used in Western Blot (WB). GTX84319
Anti-IDH1 antibody [2H9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84319
Anti-IDH1 antibody [2H9] used in Western Blot (WB). GTX84319
Anti-IDH1 antibody [2H9] used in Western Blot (WB). GTX84319

Cat. No. GTX84319

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2H9

Isotype

 IgG1

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($539)
See all IDH1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length protein expressed in 293T cell transfected with human IDH1 expression vector

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

isocitrate dehydrogenase (NADP(+)) 1, cytosolic , HEL-216 , HEL-S-26 , IDCD , IDH , IDP , IDPC , PICD

Cellular Localization

Cytoplasm, cytosol

Background

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IDH1 antibody [2H9] used in Western Blot (WB). GTX84319

GTX84319 WB Image

Overexpression

WB analysis of HEK293T cells transfected with IDH1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84319 IDH1 antibody [2H9].
Loading : 5 ug per lane

Anti-IDH1 antibody [2H9] used in Western Blot (WB). GTX84319

GTX84319 WB Image

WB analysis of various cell lines using GTX84319 IDH1 antibody [2H9].
Loading : 35 ug per lane

Anti-IDH1 antibody [2H9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84319

GTX84319 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with IDH1 plasmid using GTX84319 IDH1 antibody [2H9].

Anti-IDH1 antibody [2H9] used in Western Blot (WB). GTX84319

GTX84319 WB Image

WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon) using GTX84319 IDH1 antibody [2H9].
Loading : 10 ug per lane
Dilution : 1:200

Anti-IDH1 antibody [2H9] used in Western Blot (WB). GTX84319

GTX84319 WB Image

KO/KD

WB analysis of wild type and IDH1 knock out HeLa cell lysate(10μg per lane) using GTX84319 IDH1 antibody [2H9].
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 539