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IFIT1 antibody [3G8]

Overexpression
Anti-IFIT1 antibody [3G8] used in Western Blot (WB). GTX84315
Anti-IFIT1 antibody [3G8] used in Western Blot (WB). GTX84315
Anti-IFIT1 antibody [3G8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84315
Anti-IFIT1 antibody [3G8] used in Western Blot (WB). GTX84315
Anti-IFIT1 antibody [3G8] used in Flow cytometry (FACS). GTX84315
Anti-IFIT1 antibody [3G8] used in IHC (Paraffin sections) (IHC-P). GTX84315
Anti-IFIT1 antibody [3G8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84315
Anti-IFIT1 antibody [3G8] used in IHC (Paraffin sections) (IHC-P). GTX84315
Anti-IFIT1 antibody [3G8] used in IHC (Paraffin sections) (IHC-P). GTX84315
Anti-IFIT1 antibody [3G8] used in IHC (Paraffin sections) (IHC-P). GTX84315

Cat. No. GTX84315

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3G8

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μl ($539)
See all IFIT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1000
ICC/IF 1:50-1:100
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human IFIT1 (NP_000899) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_10729631

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interferon induced protein with tetratricopeptide repeats 1 , C56 , G10P1 , IFI-56 , IFI-56K , IFI56 , IFIT-1 , IFNAI1 , ISG56 , P56 , RNM561

Cellular Localization

Cytoplasm

Database

Research Area

DATA IMAGES

Anti-IFIT1 antibody [3G8] used in Western Blot (WB). GTX84315

GTX84315 WB Image

WB analysis of various cell lines using GTX84315 IFIT1 antibody [3G8].
Loading : 35 ug per lane

Anti-IFIT1 antibody [3G8] used in Western Blot (WB). GTX84315

GTX84315 WB Image

WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX84315 IFIT1 antibody [3G8].
Loading : 10 ug per lane
Dilution : 1:200

Anti-IFIT1 antibody [3G8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84315

GTX84315 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with IFIT1 plasmid using GTX84315 IFIT1 antibody [3G8].

Anti-IFIT1 antibody [3G8] used in Western Blot (WB). GTX84315

GTX84315 WB Image

Overexpression

WB analysis of HEK293T cells transfected with IFIT1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84315 IFIT1 antibody [3G8].
Loading : 5 ug per lane

Anti-IFIT1 antibody [3G8] used in Flow cytometry (FACS). GTX84315

GTX84315 FACS Image

FACS analysis of HEK293T cells transfected with either IFIT1 plasmid(Red) or empty vector control plasmid(Blue) using GTX84315 IFIT1 antibody [3G8].

Anti-IFIT1 antibody [3G8] used in IHC (Paraffin sections) (IHC-P). GTX84315

GTX84315 IHC-P Image

IHC-P analysis of human liver carcinoma tissue using GTX84315 IFIT1 antibody [3G8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-IFIT1 antibody [3G8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84315

GTX84315 ICC/IF Image

ICC/IF analysis of HT29 cells using GTX84315 IFIT1 antibody [3G8].

Anti-IFIT1 antibody [3G8] used in IHC (Paraffin sections) (IHC-P). GTX84315

GTX84315 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84315 IFIT1 antibody [3G8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-IFIT1 antibody [3G8] used in IHC (Paraffin sections) (IHC-P). GTX84315

GTX84315 IHC-P Image

IHC-P analysis of human liver tissue using GTX84315 IFIT1 antibody [3G8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-IFIT1 antibody [3G8] used in IHC (Paraffin sections) (IHC-P). GTX84315

GTX84315 IHC-P Image

IHC-P analysis of human kidney tissue using GTX84315 IFIT1 antibody [3G8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

REFERENCE

Application Reference

Nandi S et al. Adv Ther (Weinh) 2021; 4 (5) Synthetic platelet microgels containing fibrin knob B mimetic motifs enhance clotting responses.
Application : IHC-P
Reactivity : Human
Wichit S et al. Int J Mol Sci 2019; 20 (7) SAMHD1 Enhances Chikungunya and Zika Virus Replication in Human Skin Fibroblasts.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 539