GeneTex
United States (US)

IGF1R antibody [33255.111]

Cat No. GTX16817

Host Mouse
Clonality Monoclonal
Clone Name 33255.111
Isotype IgG1
Application WB, ELISA, Neut
Reactivity Human
APPLICATION

Application Note

For ELISA: Use at a concentration of 2 - 2.5μg/ml. For Neut: Use at a concentration of 10 - 12μg/ml. Neutralizes ~50-75% of IGF-I receptor-mediated rhIGF-I biological activity in MCF7 cells. Will block cell surface IGF-I receptor mediated IGF-I and IGF-II bioactivities. For WB: Use at a concentration of 1 - 2 μg/ml under non reducing conditions. Predicted molecular weight: 155 kDa. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

155 kDa. ( Note )

Positive Control

Recombinant IGFI or MCF7 cells

Specificity/Sensitivity

Does not cross-react with human recombinant IGFI bp1, human recombinant IGFI and human recombinant IGFII.
PROPERTIES

Form

Liquid

Buffer

From PBS, 20 mM ammonium bicarbonate, =5% trehalose, 100 μg BSA Reconstitute with sterile PBS to the desired concentration.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

recombinant human IGFIR Glu31-Asn932 of accession # P08069

Purification

Protein G affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Insulin Like Growth Factor 1 Receptor,Cd221,Igfir,Igfr,Jtk13,Igf1R

Cellular Localization

Plasma membrane

Background

This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]

Database

Research Area

Package List Price ($)
$ 339