GeneTex
United States (US)

IGFBP1 recombinant protein

Cat No. GTX65123

Application ELISA, Functional Assay, Apuri, Blocking
Reactivity Human
Species Human
APPLICATION

Application Note

The ED50 as determined by its ability to inhibit IGF-I induced proliferation of NUC-1 cells is ~100 ng/ml.

Calculated MW

28.8 kDa. ( Note )
PROPERTIES

Form

Lyophilized powder

Buffer

10 mM HCl.

Storage

Reconstitute in water to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and store at 4ºC for 1 week or –20ºC for future use.

Antigen Species

Human

Expression System

Nso cells

Purification


Purity was assessed by SDS-PAGE (≥98%) and by HPLC (≥98%).

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Insulin Like Growth Factor Binding Protein 1,Afbp,Ibp1,Igf-Bp25,Pp12,Higfbp-1,Igfbp1

Background

Human IGF-BP1 is a 25.4 kDa cysteine-rich secreted protein expressed in liver, decidual, and kidneys and is the most abundant IGF-BP in amniotic fluid. Levels of IGF-BP1 in serum are lowest after food. IGF-BP1 binds both IGF-I and IGF-II with equal affinity. Phosphorylated IGF-BP1 hinders IGF actions, whereas nonphosphorylated IGF-BP1 is stimulatory. Recombinant human IGF-BP1 is a single, glycosylated protein consisting of 218 amino acid residues.

Research Area

Package List Price ($)
$ 259