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IKB alpha antibody

KO/KD
Anti-IKB alpha antibody used in Western Blot (WB). GTX35198
Anti-IKB alpha antibody used in Immunoprecipitation (IP). GTX35198
Anti-IKB alpha antibody used in Western Blot (WB). GTX35198

Cat. No. GTX35198

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all IKB alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IP 1:20 - 1:50
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-317 of human NFKBIA (NP_065390.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NFKB inhibitor alpha , EDAID2 , IKBA , MAD-3 , NFKBI

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-IKB alpha antibody used in Western Blot (WB). GTX35198

GTX35198 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) 293T cell lysate using GTX35198 IKB alpha antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-IKB alpha antibody used in Immunoprecipitation (IP). GTX35198

GTX35198 IP Image

IP analysis of A549 cell lysate using GTX35198 IKB alpha antibody.
Antibody amount : 3μg / 150μg lysate
Dilution : 1:500

Anti-IKB alpha antibody used in Western Blot (WB). GTX35198

GTX35198 WB Image

WB analysis of various sample lysates using GTX35198 IKB alpha antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399