GeneTex
United States (US)

IKB alpha antibody

KO/KD
GTX35198_20180822_WB_1_18121410_948.jpg
GTX35198_20180822_WB_18121410_262.jpg
GTX35198_20180822_IP_18121410_666.jpg
GTX35198_20180822_ICCIF_18121410_268.jpg

Cat No. GTX35198

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:20 - 1:50
IP 1:20 - 1:50
Not tested in other applications.

Calculated MW

36 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 50% glycerol, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-317 of human NFKBIA (NP_065390.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nfkb Inhibitor Alpha,Ikba,Mad-3,Nfkbi,Nfkbia

Background

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]
DATA IMAGES
GTX35198_20180822_WB_1_18121410_948.jpg

GTX35198 WB Image

KO/KD

WB analysis of wild type and IKB alpha knockout cell lysate using GTX35198 IKB alpha antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX35198_20180822_WB_18121410_262.jpg

GTX35198 WB Image

KO/KD

WB analysis of various samples using GTX35198 IKB alpha antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX35198_20180822_IP_18121410_666.jpg

GTX35198 IP Image

KO/KD

IP analysis of A549 cell lysate using GTX35198 IKB alpha antibody.
IP reaction : 3μg antibody / 150μg cell lysate
Dilution : 1:500

GTX35198_20180822_ICCIF_18121410_268.jpg

GTX35198 ICC/IF Image

KO/KD

ICC/IF ananlysis of HeLa cells using GTX35207 RAB5A antibody.
Blue : DAPI
Dilution : 1:100

Package List Price ($)
$ 329