GeneTex

  • Country / Location Selection

United States (US)

IKB alpha (phospho Ser32/Ser36) antibody

Orthogonal
Anti-IKB alpha (phospho Ser32/Ser36) antibody used in Western Blot (WB). GTX25682

Cat. No. GTX25682

Reference 9

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Pig
Package
50 μl ($399)
See all IKB alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human IkBa that contains serines 32 and 36. The sequence is conserved in mouse and pig.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_380650

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NFKB inhibitor alpha , EDAID2 , IKBA , MAD-3 , NFKBI

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-IKB alpha (phospho Ser32/Ser36) antibody used in Western Blot (WB). GTX25682

GTX25682 WB Image

Orthogonal

WB analysis of ALLN pretreated Jurkat cells left unstimulated (Lane 1, 2) or stimulated with TNF-alpha (Lane 3-7) using GTX25682 IKB alpha (phospho Ser32/Ser36) antibody.

REFERENCE

Application Reference

Wang X et al. Bioengineered 2022; 13 (4) : 10098-10110 Adiponectin inhibits the activation of lung fibroblasts and pulmonary fibrosis by regulating the nuclear factor kappa B (NF-κB) pathway.
Application : WB
Reactivity : Human
Chao CC et al. Cancers (Basel) 2020; 12 (2) CXCL1/CXCR2 Paracrine Axis Contributes to Lung Metastasis in Osteosarcoma.
Application : WB
Reactivity : Human
Xie MY et al. J Agric Food Chem 2019; 67 (34) : 9477-9491 Porcine Milk Exosome MiRNAs Attenuate LPS-Induced Apoptosis through Inhibiting TLR4/NF-庥B and p53 Pathways in Intestinal Epithelial Cells.
Application : WB
Reactivity : Pig
Wang Z et al. Int Immunopharmacol 2019; 76 : 105873 Butylphthalide ameliorates airway inflammation and mucus hypersecretion via NF-庥B in a murine asthma model.
Wang X et al. Scand J Immunol 2019; : e12791 Epoxyeicosatrienoic acids alleviate methionine-choline-deficient diet-induced non-alcoholic steatohepatitis in mice.
Application : WB
Reactivity : Mouse
Show More +
Submit a Reference

REVIEW

There are currently no reviews for IKB alpha (phospho Ser32/Ser36) antibody (GTX25682). Be the first to share your experience with this product.
Submit a Review
Application Reference
Wang X et al. Bioengineered 2022; 13 (4):10098-10110 Adiponectin inhibits the activation of lung fibroblasts and pulmonary fibrosis by regulating the nuclear factor kappa B (NF-κB) pathway.
Application : WB
Reactivity : Human
Chao CC et al. Cancers (Basel) 2020; 12 (2) CXCL1/CXCR2 Paracrine Axis Contributes to Lung Metastasis in Osteosarcoma.
Application : WB
Reactivity : Human
Xie MY et al. J Agric Food Chem 2019; 67 (34):9477-9491 Porcine Milk Exosome MiRNAs Attenuate LPS-Induced Apoptosis through Inhibiting TLR4/NF-庥B and p53 Pathways in Intestinal Epithelial Cells.
Application : WB
Reactivity : Pig
Wang Z et al. Int Immunopharmacol 2019; 76:105873 Butylphthalide ameliorates airway inflammation and mucus hypersecretion via NF-庥B in a murine asthma model.
Wang X et al. Scand J Immunol 2019;:e12791 Epoxyeicosatrienoic acids alleviate methionine-choline-deficient diet-induced non-alcoholic steatohepatitis in mice.
Application : WB
Reactivity : Mouse
Lee HM et al. Transplant Proc 2016; 48 (4):1226-33 Protective Effect of Eupatilin Pretreatment Against Hepatic Ischemia-Reperfusion Injury in Mice.
Cho EC et al. Mediators Inflamm 2015; 2015:287345 RRM2B-Mediated Regulation of Mitochondrial Activity and Inflammation under Oxidative Stress.
Application : WB
Reactivity : Human
Previtera ML et al. PLoS One 2015; 10 (12):e0145813 Substrate Stiffness Regulates Proinflammatory Mediator Production through TLR4 Activity in Macrophages.
Application : WB
Reactivity : Mouse
Hung CJ et al. J Endod 2013; 39 (12):1557-61 Antiosteoclastogenic activity of silicate-based materials antagonizing receptor activator for nuclear factor kappaB ligand-induced osteoclast differentiation of murine marcophages.
Application : WB
Reactivity : Mouse
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399