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IKK alpha antibody [14A231]

Anti-IKK alpha antibody [14A231] used in Western Blot (WB). GTX12138
Anti-IKK alpha antibody [14A231] used in Western Blot (WB). GTX12138
Anti-IKK alpha antibody [14A231] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX12138
Anti-IKK alpha antibody [14A231] used in Flow cytometry (FACS). GTX12138
Anti-IKK alpha antibody [14A231] used in Western Blot (WB). GTX12138
Anti-IKK alpha antibody [14A231] used in IHC (Paraffin sections) (IHC-P). GTX12138

Cat. No. GTX12138

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 14A231

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ChIP assay

Reactivity

 Human, Mouse, Rat, Primate
Package
50 μg ($399)
See all IKK alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/ml
ICC/IF 1:10
IHC-P 5 μg/ml
IHC-Fr Assay dependent
FACS 0.25 - 0.5 μg / 10⁶ cells
IP 1 - 2 μg/ml
ChIP assay 1:10 - 1:500
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Predict Reactivity

Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This antibody was raised against a His-tagged full-length human IKK alpha protein.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

component of inhibitor of nuclear factor kappa B kinase complex , IKBKA , IKK-alpha , IKK1 , IKKA , NFKBIKA , TCF16

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IKK alpha antibody [14A231] used in Western Blot (WB). GTX12138

GTX12138 WB Image

WB analysis of Daudi (left) and U937 (right) cell lysate using GTX12138 IKK alpha antibody [14A231].

Anti-IKK alpha antibody [14A231] used in Western Blot (WB). GTX12138

GTX12138 WB Image

WB analysis of multiple samples using GTX12138 IKK alpha antibody [14A231].
Dilution : 1 μg/ml

Anti-IKK alpha antibody [14A231] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX12138

GTX12138 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX12138 IKK alpha antibody [14A231].
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 1:10

Anti-IKK alpha antibody [14A231] used in Flow cytometry (FACS). GTX12138

GTX12138 FACS Image

FACS (Intracellular staining) analysis of HEK293 cells using GTX12138 IKK alpha antibody [14A231].
Red : primary antibody
Green : isotype control
Antibody amount : 0.5μg

Anti-IKK alpha antibody [14A231] used in Western Blot (WB). GTX12138

GTX12138 WB Image

WB analysis of Daudi cell lysate using GTX12138 IKK alpha antibody [14A231].
Dilution : 1 μg/ml

Anti-IKK alpha antibody [14A231] used in IHC (Paraffin sections) (IHC-P). GTX12138

GTX12138 IHC-P Image

IHC-P analysis of human kidney tissue using GTX12138 IKK alpha antibody [14A231].
Dilution : 5 μg/ml
Antigen retrieval : 10 mM sodium citrate buffer, pH 6.0

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399