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IL1 alpha antibody

Orthogonal
Anti-IL1 alpha antibody used in Western Blot (WB). GTX113088
Anti-IL1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX113088
Anti-IL1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113088
Anti-IL1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX113088

Cat. No. GTX113088

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all IL1 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.09 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IL1 alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11175809

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 1 alpha , IL-1A , IL1 , IL1-ALPHA , IL1F1

Cellular Localization

Secreted

Background

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IL1 alpha antibody used in Western Blot (WB). GTX113088

GTX113088 WB Image

Orthogonal

Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 alpha antibody (GTX113088) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IL1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX113088

GTX113088 IHC-P Image

IL1 alpha antibody detects IL1 alpha protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
IL1 alpha stained by IL1 alpha antibody (GTX113088) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IL1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113088

GTX113088 ICC/IF Image

IL1 alpha antibody detects IL1 alpha protein at cytoplasm by immunofluorescent analysis.
Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IL1 alpha protein stained by IL1 alpha antibody (GTX113088) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-IL1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX113088

GTX113088 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using IL1 alpha(GTX113088) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169