GeneTex
United States (US)

IL10 recombinant protein

Cat No. GTX65348

Application ELISA, Functional Assay, Apuri, Blocking
Reactivity Mouse
Species Mouse
APPLICATION

Application Note

The ED50 determined by the dose-dependent co-stimulation (with murine IL-4) of MC/9 cells is <2 ng/ml, corresponding to a specific activity of > 5 x10^5 units/mg.

Calculated MW

18.7 kDa. ( Note )
PROPERTIES

Form

Lyophilized powder

Buffer

3 mM Tris, pH 8.0

Storage

Reconstitute in 3 mM Tris, pH 8.0 to a concentration of 0.1-1.0 μg/μl. The solution can then be diluted into other aqueous buffers and stored at 4ºC for 1 week or –20ºC for future use.

Antigen Species

Mouse

Expression System

E. coli

Purification


Purity was assessed by SDS-PAGE (≥98%) and by HPLC (≥98%).

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Interleukin 10,Csif,Il-10,Il10

Background

IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. IL-10 inhibits the expression of proinflammatory cytokines such as IL-1 and TNF-?N. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions. Human IL-10 active on murine cells, but murine IL-10 is inactive on human cells. Recombinant murine IL-10 is an 18.7 kDa protein of 161 amino acid residues.

Research Area

Package List Price ($)
$ 599