APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.1-0.2 μg/ml |
0.5-1.0 μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
recombinant human IL-12 Rβ1
Product Note
The antibody shows 5% cross-reactivity with recombinant mouse IL-12 Rβ1.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
recombinant human IL-12 Rβ1 extracellular domain, expressed in NSO cells
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
interleukin 12 receptor subunit beta 1 , CD212 , IL-12R-BETA1 , IL12RB , IMD30
Cellular Localization
Membrane; Single-pass type I membrane protein
Background
Interleukin 12 (IL12) was identified as a factor secreted by human Epstein-Barr (EBV) transformed B cell lines. IL12, or Natural Killer Cell Stimulatory Factor (NKSF), is a 75 kDa disulfide-linked heterodimer of a 35 kDa subunit and 40 kDa subunit. IL12 is produced predominantly by monocytes and NK cells2 and induces T cells and NK cells to produce IFN gamma. Human IL12 is not active on mouse cells, but murine IL12 is active on both murine and human lymphocytes. IL12 signals through the IL12 receptor, which is composed of at least two subunits designated beta1 and beta2. Both IL12 receptor subunits are members of the hemapoietin receptor superfamily and have strong homology to the gp130 receptor. The beta1 receptor, although a low affinity binder of IL12, is not capable of transducing an IL12-mediated signal. A second subunit of the IL12 receptor was subsequently identified that when coexpressed with the beta1 subunit forms a high affinity receptor for IL12 and confers IL12 signaling.
Database
Research Area
REFERENCE
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REVIEW
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