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IL17A antibody

Orthogonal
Anti-IL17A antibody used in Western Blot (WB). GTX32674
Anti-IL17A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32674

Cat. No. GTX32674

Reference 1
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all IL17A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 24-155 of human IL17A (NP_002181.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887673

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 17A , CTLA-8 , CTLA8 , IL-17 , IL-17A , IL17

Cellular Localization

Secreted

Background

The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-IL17A antibody used in Western Blot (WB). GTX32674

GTX32674 WB Image

Orthogonal

WB analysis of THP-1 cells treated by PMA (80 nM) at 37ºC for overnight using GTX32674 IL17A antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-IL17A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32674

GTX32674 ICC/IF Image

ICC/IF analysis of L929 cells using GTX32674 IL17A antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

Application Reference

Liu SP et al. CNS Neurosci Ther 2019; (Epub) Secukinumab attenuates reactive astrogliosis via IL-17RA/(C/EBPβ)/SIRT1 pathway in a rat model of germinal matrix hemorrhage.
Application : WB
Reactivity : Rat
Submit a Reference

REVIEW

IL17A antibody Cat. No. GTX32674

Rating
( Average 3 based on 1 users reviews)
Application
IHC (Frozen sections)(IHC-Fr)
( Average 3 based on 1 users reviews)
Anti-IL17A antibody used in IHC (Frozen sections) (IHC-Fr). GTX32674
Date : Anonymous submitted on 26-May-2017
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : Spleen
Blocking : 1XPBS+ 0.3% Triton? X-100 + 1%BSA+human serum, 25°C, 0.5Hr
Primary Antibody : 1:30, 4°C, 16Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399