APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 μg/mL |
| 10 μg/mL |
| 5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Raji Cell Slide(GTX25538) , Raji Cell Lysate(GTX27908) , Human Pancreas Tissue Slide
Product Note
This antibody is specific for IL23 C-Terminus
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
IL-23 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human IL-23.The immunogen is located within the last 50 amino acids of IL-23.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
interleukin 23 subunit alpha , IL-23 , IL-23A , IL23P19 , P19 , SGRF
Cellular Localization
Secreted
Background
Like interleukin-27 (IL-27), IL-23 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the IL-12p40 subunit and a novel p19 subunit. IL-23 is secreted by activated dendritic cells, macrophages, and monocytes. Its biological activities include enhancing the proliferation of memory T cells and the production of IFN-gamma, IL-12, and TNF-alpha from activated T cells, and can stimulate macrophages to produce TNF-alpha and nitric oxide. It has also been shown to possess potent anti-tumor and anti-metastatic activity in mouse models of cancer, suggesting a potential role for IL-23 in therapeutic treatment of cancer.
Database
Research Area
DATA IMAGES
|
GTX85496 WB Image
WB analysis of human IL23 recombinant protein using GTX85496 IL23 antibody.
Dilution : 4μg/ml
Loading : 30ng
|
|
|
GTX85496 IHC-P Image
IHC-P analysis of human pancreas tissue using GTX85496 IL23 antibody.
Red : Primary antibody
Blue : DAPI
Dilution : 20 μg/ml
|
|
|
GTX85496 WB Image
WB analysis of various samples using GTX85496 IL23 antibody.
Dilution : 2μg/ml
Loading : 20μg
|
|
|
GTX85496 ICC/IF Image
Immunofluorescence of IL-23 in Raji cells with IL-23 antibody at 20 ug/mL.
|
|
|
GTX85496 ICC/IF Image
Immunocytochemistry of IL-23 in Raji cells with IL-23 antibody at 10 ug/ml.
|
|
|
GTX85496 WB Image
Western blot analysis of IL-23 in Raji lysate with IL-23 antibody at 1 ug/ml in the (A) absence and (B) presence of blocking peptide.
|
REFERENCE
There are currently no references for IL23 antibody (GTX85496). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for IL23 antibody (GTX85496). Be the first to share your experience with this product.
