GeneTex
United States (US)

IL4 recombinant protein

Cat No. GTX65359

Application ELISA, Functional Assay, Apuri, Blocking
Reactivity Mouse
Species Mouse
APPLICATION

Application Note

The ED50 determined by the dose-dependent stimulation of the proliferation of murine HT-2 cells is <.2.0 ng/ml, corresponding to a specific activity of >5 x10^5 units/mg.

Calculated MW

13.5 kDa. ( Note )
PROPERTIES

Form

Lyophilized powder

Storage

Reconstitute in water to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4ºC for 1 week or –20ºC for future use.

Antigen Species

Mouse

Expression System

E. coli

Purification


Purity was assessed by SDS-PAGE (≥98%) and by HPLC (≥98%).

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

BCGF, B cell stimulating factor, Lymphocyte stimulatory factor 1, MGC79402, Binetrakin, Pitrakinra, IL4, BSF1, BCDF, Lymphocyte stimulatory factor1, BSF-1, IL-4, IL 4, BSF 1

Background

IL-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Produced by mast cells, T cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy. Recombinant murine IL-4 is a 13.5 kDa globular protein containing 120 amino acid residues.

Research Area

Package List Price ($)
$ 249