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ILF3 antibody

Anti-ILF3 antibody used in Western Blot (WB). GTX55155

Cat. No. GTX55155

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399)
See all ILF3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
Not tested in other applications.

Calculated MW

95 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of ILF3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887917

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin enhancer binding factor 3 , CBTF , DRBF , DRBP76 , MMP4 , MPHOSPH4 , MPP4 , NF-AT-90 , NF110 , NF110b , NF90 , NF90a , NF90b , NFAR , NFAR-1 , NFAR2 , TCP110 , TCP80

Cellular Localization

Cytoplasm,Nucleus,Nucleolus

Background

This gene encodes a double-stranded RNA (dsRNA) binding protein that complexes with other proteins, dsRNAs, small noncoding RNAs, and mRNAs to regulate gene expression and stabilize mRNAs. This protein (NF90, ILF3) forms a heterodimer with a 45 kDa transcription factor (NF45, ILF2) required for T-cell expression of interleukin 2. This complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. In contrast, an isoform (NF110) of this gene that is predominantly restricted to the nucleus has only minor effects on cell growth when its levels are reduced. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Dec 2014]

Database

Research Area

DATA IMAGES

Anti-ILF3 antibody used in Western Blot (WB). GTX55155

GTX55155 WB Image

WB analysis of A431 (A), Raji (B), K562 (C) whole cell lysates using GTX55155 ILF3 antibody.

REFERENCE

Application Reference

Todaka H et al. Mol Cell Biol 2015; 35 (13) : 2295-308 Overexpression of NF90-NF45 Represses Myogenic MicroRNA Biogenesis, Resulting in Development of Skeletal Muscle Atrophy and Centronuclear Muscle Fibers.
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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399